LOCALIZATION AND QUANTIFICATION OF EPIDERMAL GROWTH-FACTOR RECEPTORS ON SINGLE CELLS BY CONFOCAL LASER SCANNING MICROSCOPY

被引：28

作者：

GOOD, MJ ^{[1
]}

HAGE, WJ ^{[1
]}

MUMMERY, CL ^{[1
]}

DELAAT, SW ^{[1
]}

BOONSTRA, J ^{[1
]}

机构：

[1] UNIV UTRECHT,DEPT MOLEC CELL BIOL,3584 CH UTRECHT,NETHERLANDS

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1992年 / 40卷 / 09期

关键词：

EPIDERMAL GROWTH FACTOR RECEPTOR; CONFOCAL LASER SCANNING MICROSCOPY; EMBRYONAL CARCINOMA CELLS; A431; CELLS; BINDING ANALYSIS;

D O I：

10.1177/40.9.1506672

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have established a method for quantifying binding of fluorescence-labeled growth factors to their receptors on single cells in situ with the confocal laser scanning microscope (CLSM). Biotinylated epidermal growth factor (EGF) coupled to phycoerythrin-labeled anti-biotin was used to compare the levels of fluorescence on three different cell types for which the number of EGF factors was known from Scatchard analysis of [I-125]-EGF binding. The results showed that as few as 10,000 receptors/cell were detectable above background. This method will provide a rapid and quantifiable alternative to autoradiography for ligand binding to single cells in situ.

引用

页码：1353 / 1361

页数：9