NMDA NEUROTOXICITY IN MURINE CORTICAL CELL-CULTURES IS NOT ATTENUATED BY HEMOGLOBIN OR INHIBITION OF NITRIC-OXIDE SYNTHESIS

被引:50
作者
REGAN, RF
RENN, KE
PANTER, SS
机构
[1] UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, DEPT NEUROSURG, SAN FRANCISCO, CA 94143 USA
关键词
NEURONAL CULTURE; EXCITATORY AMINO ACID; NITRIC OXIDE; HEMOGLOBIN; CALCIUM; N-METHYL-D-ASPARTATE; CYCLIC GUANOSINE MONOPHOSPHATE;
D O I
10.1016/0304-3940(93)90075-V
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The role of nitric oxide in N-methyl-D-aspartate (NMDA) neurotoxicity was investigated in murine cortical cell cultures. Exposure of cultures to 300 muM NMDA for 5 min resulted in death of 50-80% of neurons over the subsequent 24 h. This injury was not attenuated by hemoglobin, the nitric oxide synthase (NOS) inhibitors N(G)monomethyl-L-arginine (MMA) or Nomega-nitro-L-arginine (NA), or L-arginine depletion. Hemoglobin and NOS inhibitors consistently prevented the increase in cyclic guanosine monophosphate (cGMP) seen after NMDA exposure. These results suggest that NMDA neurotoxicity in this cell culture system is mediated, at least in part, by mechanisms other than NOS activation.
引用
收藏
页码:53 / 56
页数:4
相关论文
共 20 条
  • [1] PILOT SCALE PRODUCTION OF PYROGEN-FREE HUMAN HEMOGLOBIN FOR RESEARCH
    CHAPMAN, KW
    SNELL, SM
    JESSE, RG
    MORANO, JK
    EVERSE, J
    WINSLOW, RM
    [J]. BIOMATERIALS ARTIFICIAL CELLS AND IMMOBILIZATION BIOTECHNOLOGY, 1992, 20 (2-4): : 415 - 421
  • [2] GLUTAMATE NEUROTOXICITY AND DISEASES OF THE NERVOUS-SYSTEM
    CHOI, DW
    [J]. NEURON, 1988, 1 (08) : 623 - 634
  • [3] CHOI DW, 1988, J NEUROSCI, V8, P185
  • [4] CHOI DW, 1987, J NEUROSCI, V7, P369
  • [5] CHOI DW, 1987, J NEUROSCI, V7, P357
  • [6] PREPARATION OF HUMAN HEMOGLOBIN-AO FOR POSSIBLE USE AS A BLOOD SUBSTITUTE
    CHRISTENSEN, SM
    MEDINA, F
    WINSLOW, RW
    SNELL, SM
    ZEGNA, A
    MARINI, MA
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1988, 17 (02): : 143 - 154
  • [7] NITRIC-OXIDE MEDIATES GLUTAMATE NEUROTOXICITY IN PRIMARY CORTICAL CULTURES
    DAWSON, VL
    DAWSON, TM
    LONDON, ED
    BREDT, DS
    SNYDER, SH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (14) : 6368 - 6371
  • [8] ABSENCE OF IMPLICATION OF L-ARGININE/NITRIC OXIDE PATHWAY ON NEURONAL CELL INJURY INDUCED BY L-GLUTAMATE OR HYPOXIA
    DEMERLEPALLARDY, C
    LONCHAMPT, MO
    CHABRIER, PE
    BRAQUET, P
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (01) : 456 - 464
  • [9] Evelyn KA, 1938, J BIOL CHEM, V126, P655
  • [10] GLUTAMATE, NITRIC-OXIDE AND CELL CELL SIGNALING IN THE NERVOUS-SYSTEM
    GARTHWAITE, J
    [J]. TRENDS IN NEUROSCIENCES, 1991, 14 (02) : 60 - 67