CLONING AND CHARACTERIZATION OF THE ADENINE PHOSPHORIBOSYLTRANSFERASE-ENCODING GENE (APT1) FROM SACCHAROMYCES-CEREVISIAE

被引：14

作者：

ALFONZO, JD

SAHOTA, A

DEELEY, MC

RANJEKAR, P

TAYLOR, MW

机构：

[1] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47405

[2] INDIANA UNIV,DEPT MED GENET,INDIANAPOLIS,IN 46223

[3] IMMUNEX CO,SEATTLE,WA 98020

[4] NATL CHEM LAB,POONA 411008,MAHARASHTRA,INDIA

来源：

GENE | 1995年 / 161卷 / 01期

关键词：

YEAST; RECOMBINANT DNA; NUCLEOTIDE SEQUENCING; GENE EXPRESSION; ADENINE UTILIZATION; PRIMER EXTENSION;

D O I：

10.1016/0378-1119(95)00239-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned, sequenced and characterized the APT1 (adenine phosphoribosyltransferase) gene from Saccharomyces cerevisiae. The APT1 sequence includes and open reading frame encoding 221 amino acids is contained within a 1322-bp insert that complements APRT-deficient mutants to wild-type levels of enzyme activity. Analysis by primer extension revealed multiple transcription start points (tsp) and a major tsp 21-bp upstream from the ATG start codon. A transcript initiated at the major tsp would yield a 700-nt mRNA which is in agreement with the size observed by Northern analysis. Sequence comparison indicates that the yeast enzyme shares strong similarities with other known APRT of bacterial, invertebrate, plant and mammalian origins.

引用

页码：81 / 85

页数：5

共 18 条

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