DEGRADATION OF THE DI-PROTEIN OF PHOTOSYSTEM-II REACTION-CENTER BY ULTRAVIOLET-B RADIATION REQUIRES THE PRESENCE OF FUNCTIONAL MANGANESE ON THE DONOR SIDE

被引：61

作者：

BARBATO, R ^{[1
]}

FRIZZO, A ^{[1
]}

FRISO, G ^{[1
]}

RIGONI, F ^{[1
]}

GIACOMETTI, GM ^{[1
]}

机构：

[1] UNIV PADUA,DIPARTIMENTO BIOL,I-35121 PADUA,ITALY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 227卷 / 03期

关键词：

PHOTOSYSTEM II; D1; PROTEIN; PHOTOINHIBITION; ULTRAVIOLET B; SPINACH;

D O I：

10.1111/j.1432-1033.1995.tb20194.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vivo effects of ultraviolet-B radiation (280-320 nm) on photosystem-II activity and degradation of the D1 protein are investigated and compared with the in vitro results on isolated thylakoids and other detergent-extracted photosystem-II preparations. A cleavage site in the second transmembrane segment of the D1 protein, giving rise to a 20-kDa C-terminal and a 13-kDa N-terminal fragment pair, is detected after irradiation of entire leaves as well as in all photosystem-II preparations, irrespective of their actual ability to evolve oxygen but depending on the presence of Mn ions associated with the water-splitting system. Damage to the plastoquinone moiety, observed by other authors, is confirmed and is proposed to be responsible for the impairment of electron-transport activity, but not for the observed cleavage of the D1 protein.

引用

页码：723 / 729

页数：7

共 59 条

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