FLUORESCENT DYE PHOSPHORAMIDITE LABELING OF OLIGONUCLEOTIDES

被引：48

作者：

THEISEN, P ^{[1
]}

MCCOLLUM, C ^{[1
]}

UPADHYA, K ^{[1
]}

JACOBSON, K ^{[1
]}

VU, HN ^{[1
]}

ANDRUS, A ^{[1
]}

机构：

[1] APPL BIOSYST INC,850 LINCOLN CTR DR,FOSTER CITY,CA 94404

来源：

TETRAHEDRON LETTERS | 1992年 / 33卷 / 35期

关键词：

D O I：

10.1016/S0040-4039(00)61181-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A 5-carboxyfluorescein (FAM) phosphoramidite reagent has been synthesized for use on automated DNA synthesizers, to prepare fluorescent dye labelled oligonucleotides. Oligonucleotides can be labelled at any site in a sequence and multiple labelled. The FAM dye can also be coupled to a polystyrene synthesis support to prepare 3' fluorescent labelled oligonucleotides.

引用

页码：5033 / 5036

页数：4