POLYMERASE CHAIN-REACTION BASED CDNA CLONING OF WHEAT PROFILIN - A POTENTIAL PLANT ALLERGEN

被引：33

作者：

RIHS, HP

ROZYNEK, P

MAYTAUBE, K

WELTICKE, B

BAUR, X

机构：

[1] Abteilung Molekularc Genetik, Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, Institut an der Ruhr-Universität, Bochum

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 105卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; PROFILIN; SEQUENCE POLYMORPHISM; WHEAT;

D O I：

10.1159/000236824

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high amino acid sequence identity with the birch pollen allergen Betv II suggests that wheat profilin may be a potential plant allergen, relevant in food allergy.

引用

页码：190 / 194

页数：5

共 24 条

[1] SECONDARY STRUCTURE AND TOPOLOGY OF ACANTHAMOEBA PROFILIN-I AS DETERMINED BY HETERONUCLEAR NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY [J].

ARCHER, SJ ;

VINSON, VK ;

POLLARD, TD ;

TORCHIA, DA .

BIOCHEMISTRY, 1993, 32 (26) :6680-6687

[2] 4 RECOMBINANT ISOFORMS OF COR ALPHA-I, THE MAJOR ALLERGEN OF HAZEL POLLEN, SHOW DIFFERENT IGE-BINDING PROPERTIES [J].

BREITENEDER, H ;

FERREIRA, F ;

HOFFMANNSOMMERGRUBER, K ;

EBNER, C ;

BREITENBACH, M ;

RUMPOLD, H ;

KRAFT, D ;

SCHEINER, O .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (02) :355-362

[3] ACTIN POLYMERIZABILITY IS INFLUENCED BY PROFILIN, A LOW-MOLECULAR WEIGHT PROTEIN IN NON-MUSCLE CELLS [J].

CARLSSON, L ;

NYSTROM, LE ;

SUNDKVIST, I ;

MARKEY, F ;

LINDBERG, U .

JOURNAL OF MOLECULAR BIOLOGY, 1977, 115 (03) :465-483

[4] SEQUENCE POLYMORPHISMS OF CDNA CLONES ENCODING THE MITE ALLERGEN DER-P-I [J].

CHUA, KY ;

KEHAL, PK ;

THOMAS, WR .

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1993, 101 (04) :364-368

[5] CHICKADEE ENCODES A PROFILIN REQUIRED FOR INTERCELLULAR CYTOPLASM TRANSPORT DURING DROSOPHILA OOGENESIS [J].

COOLEY, L ;

VERHEYEN, E ;

AYERS, K .

CELL, 1992, 69 (01) :173-184

[6] THE PRIMARY STRUCTURE OF TETRAHYMENA PROFILIN [J].

EDAMATSU, M ;

HIRONO, M ;

TAKEMASA, T ;

WATANABE, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 175 (02) :543-550

[7] THE ACTIN-BINDING PROTEIN PROFILIN BINDS TO PIP2 AND INHIBITS ITS HYDROLYSIS BY PHOSPHOLIPASE-C [J].

GOLDSCHMIDTCLERMONT, PJ ;

MACHESKY, LM ;

BALDASSARE, JJ ;

POLLARD, TD .

SCIENCE, 1990, 247 (4950) :1575-1578

[8] REGULATION OF PHOSPHOLIPASE-C-GAMMA-1 BY PROFILIN AND TYROSINE PHOSPHORYLATION [J].

GOLDSCHMIDTCLERMONT, PJ ;

KIM, JW ;

MACHESKY, LM ;

RHEE, SG ;

POLLARD, TD .

SCIENCE, 1991, 251 (4998) :1231-1233

[9] SEQUENCE POLYMORPHISM OF AMB-A-I AND AMB-A-II, THE MAJOR ALLERGENS IN AMBROSIA-ARTEMISIIFOLIA (SHORT RAGWEED) [J].

GRIFFITH, IJ ;

POLLOCK, J ;

KLAPPER, DG ;

ROGERS, BL ;

NAULT, AK .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1991, 96 (04) :296-304

[10] MUTATIONAL ANALYSIS OF YEAST PROFILIN [J].

HAARER, BK ;

PETZOLD, AS ;

BROWN, SS .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (12) :7864-7873

← 1 2 3 →