Hepatic levels of several metabolites of the Embden‐Meyerhof pathway, the citric acid cycle and oxidized and reduced substrate pairs of the lactate, α‐glycerophosphate, β‐hydroxybutyrate, malate, and glutamate dehydrogenase systems were measured in freeze clamped livers from fed male rats, before and after ethanol infusion. Ethanol infusion in fed male rats after 30 min resulted in an increase in the levels of hexosemonophosphates, triosephosphates, fructose 1,6 diphosphate, lactate, α‐glycerophosphate, malate, β‐hydroxybutyrate, and glutamate. [Lactate]/[pyruvate] ratio increased about 3 fold, [α‐glycerophosphate]/[dihydroxyacetonephosphate] 1.5 fold, [malate]/[oxaloacetate], [β‐hydroxybutyrate]/[acetoacetate], and [glutamate]/[2‐oxoglutarate] × [NH4+] about 2 fold. The levels of pyruvate, acetyl CoA, citrate, and ATP were decreased during ethãnol treatment. The hepatic levels of glycogen, oxaloacetate, 2‐oxoglutarate, ammonia, aspartate, and acetoacetate remained unchanged, 30 min after ethanol infusion, as compared to values before ethanol infusion. Hepatic glucose 6‐phosphatase activity was significantly increased after 15 min of ethanol infusion (1.5 g/kg body weight). The glucose 6‐phosphatase activity showed a maximum 15 min after ethanol infusion, the fructose 1,6 diphosphatase activity did not change significantly 15 and 30 min after ethanol infusion. Hepatic acid and alkaline phosphatase activities measured with β‐glycerophosphate as substrate showed no significant changes compared to values from normal rat livers. The presence of ethanol (8 mM) in the incubation medium showed no significant effect on the [6‐14C]glucose incorporation into glycogen by the liver slices. Copyright © 1968, Wiley Blackwell. All rights reserved
