THE DETECTION OF ANTI-RO/SS-A AND ANTI-LA/SS-B ANTIBODIES - A COMPARISON OF COUNTERIMMUNOELECTROPHORESIS WITH IMMUNOBLOT, ELISA, AND RNA-PRECIPITATION ASSAYS

被引：62

作者：

MEILOF, JF ^{[1
]}

BANTJES, I ^{[1
]}

DEJONG, J ^{[1
]}

VANDAM, AP ^{[1
]}

SMEENK, RJT ^{[1
]}

机构：

[1] NETHERLANDS RED CROSS, BLOOD TRANSFUS SERV, CENT LAB, 1066 CX AMSTERDAM, NETHERLANDS

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 133卷 / 02期

关键词：

Anti-La/SS-B; Anti-Ro/SS-A; Counterimmunoelectrophoresis; ELISA; Immunoblotting; RNA precipitation;

D O I：

10.1016/0022-1759(90)90362-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence in serum of anti-Ro/SS-A and/or anti-La/SS-B autoantibodies is a characteristic of autoimmune diseases such as Sjögren's syndrome and systemic lupus erythematosus. To evaluate different assays currently available for the detection of these antibodies 50 sera were tested using the four different assay methods: counterimmunoelectrophoresis (CIE), RNA precipitation assay, immunoblotting technique and ELISA. The RNA-precipitation assay showed the highest sensitivity and specificity. The CIE for the detection of anti-Ro/SS-A antibodies gave comparable results whereas the Ro/SS-A ELISA and Ro/SS-A or HeLa immunoblot showed lower sensitivities (96% and 80% respectively). Sensitivity was even lower (66%) when only reactivity towards the 60 kDa Ro/SS-A protein was considered. The ELISA for the detection of anti-La/SS-B antibodies showed a sensitivity of 98%, the immunoblotting technique of 86% and the CIE only 67%. The high sensitivity of the La/SS-B ELISA went together with a low specificity of 14%. We conclude from these data that for the detection of anti-Ro/SS-A and anti-La/SS-B antibodies the RNA precipitation assay shows the highest sensitivity and highest specificity. For routine screening purposes the CIE is the most convenient and reliable assay to detect anti-Ro/SS-A antibodies. For the detection of anti-La/SS-B antibodies the immunoblot corresponds most closely to the RNA precipitation assay. © 1990.

引用

页码：215 / 226

页数：12

共 36 条

[11] DETECTION OF ANTIBODIES TO SMALL NUCLEAR RIBONUCLEOPROTEINS AND SMALL CYTOPLASMIC RIBONUCLEOPROTEINS USING UNLABLED CELL-EXTRACTS [J].

FORMAN, MS ;

NAKAMURA, M ;

MIMORI, T ;

GELPI, C ;

HARDIN, JA .

ARTHRITIS AND RHEUMATISM, 1985, 28 (12) :1356-1361

[12] FUNCTION OF THE MAMMALIAN LA PROTEIN - EVIDENCE FOR ITS ACTION IN TRANSCRIPTION TERMINATION BY RNA POLYMERASE-III [J].

GOTTLIEB, E ;

STEITZ, JA .

EMBO JOURNAL, 1989, 8 (03) :851-861

[13] SS-B/LA ANTIGEN PURIFICATION, AND ELISA DETECTION OF ANTI-SS-B/LA ANTIBODIES IN SERA FROM PATIENTS WITH INFLAMMATORY CONNECTIVE-TISSUE DISEASES [J].

HANSEN, B ;

LARSEN, P ;

MANTHORPE, R .

ALLERGY, 1988, 43 (08) :577-585

[14]

HARLEY JB, 1984, J RHEUMATOL, V11, P309

[15] RO SMALL CYTOPLASMIC RIBONUCLEOPROTEINS ARE A SUBCLASS OF LA RIBONUCLEOPROTEINS - FURTHER CHARACTERIZATION OF THE RO AND LA SMALL RIBONUCLEOPROTEINS FROM UNINFECTED MAMMALIAN-CELLS [J].

HENDRICK, JP ;

WOLIN, SL ;

RINKE, J ;

LERNER, MR ;

STEITZ, JA .

MOLECULAR AND CELLULAR BIOLOGY, 1981, 1 (12) :1138-1149

[16] IDENTIFICATION OF ANTIBODIES TO NUCLEAR ACIDIC ANTIGENS BY COUNTERIMMUNOELECTROPHORESIS [J].

KURATA, N ;

TAN, EM .

ARTHRITIS AND RHEUMATISM, 1976, 19 (03) :574-580

[17] 2 NOVEL CLASSES OF SMALL RIBONUCLEOPROTEINS DETECTED BY ANTIBODIES ASSOCIATED WITH LUPUS-ERYTHEMATOSUS [J].

LERNER, MR ;

BOYLE, JA ;

HARDIN, JA ;

STEITZ, JA .

SCIENCE, 1981, 211 (4480) :400-402

[18] ANTIBODIES TO SMALL NUCLEAR RNAS COMPLEXED WITH PROTEINS ARE PRODUCED BY PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS [J].

LERNER, MR ;

STEITZ, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (11) :5495-5499

[19]

LIEU TS, 1984, J IMMUNOL METHODS, V71, P217

[20]

LIEU TS, 1989, CLIN RES, V37, pA352

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