TRACING AND IDENTIFICATION OF URICASE REACTION INTERMEDIATES - A DIRECT C-13-NMR ISOTOPE-LABELING EVIDENCE

被引：38

作者：

MODRIC, N

DEROME, AE

ASHCROFT, SJH

POJE, M

机构：

[1] UNIV ZAGREB, FAC SCI, ORGAN CHEM LAB, POB 153, YU-41001 ZAGREB, CROATIA

[2] UNIV OXFORD, DYSON PERRINS LAB, OXFORD OX1 3QY, ENGLAND

[3] UNIV OXFORD, NUFFIELD DEPT CLIN BIOCHEM, OXFORD, ENGLAND

来源：

TETRAHEDRON LETTERS | 1992年 / 33卷 / 44期

关键词：

D O I：

10.1016/S0040-4039(00)61021-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The C-13-NMR spectra are characteristic and provide a convenient method to chart the pathway of uricase-catalysed oxidation of [5-C-13]urate (1) to [4-C-13]allantoin (5); 5-hydroxy[5-C-13]isouric acid (2), its ring-opened derivative 3, and [4-C-13]allantoin-5-carboxylate (4) were identified as consecutive intermediates in D2O/phosphate buffer (pD 7.2).

引用

页码：6691 / 6694

页数：4

共 19 条

[11] THE MECHANISM FOR THE CONVERSION OF URIC-ACID INTO ALLANTOIN AND DEHYDRO-ALLANTOIN - A NEW LOOK AT AN OLD PROBLEM [J].