PREPARATION AND CHARACTERIZATION OF A S-MONOMETHOXYPOLY-(ETHYLENE GLYCOL) THIODERIVATIVE OF PAPAIN

被引：14

作者：

PAUL, C ^{[1
]}

VINCENTELLI, J ^{[1
]}

BRYGIER, J ^{[1
]}

MUSU, T ^{[1
]}

BAEYENSVOLANT, D ^{[1
]}

GUERMANT, C ^{[1
]}

LOOZE, Y ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,FAC MED,PROTEIN CHEM UNIT,B-1050 BRUSSELS,BELGIUM

来源：

PHYTOCHEMISTRY | 1994年 / 35卷 / 06期

关键词：

CARICA PAPAYA; CARICACEAE; PROTEINASES; CYSTEINE-PROTEINASES; PEG; COVALENT CHROMATOGRAPHY; AFFINITY CHROMATOGRAPHY;

D O I：

10.1016/S0031-9422(00)86866-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A derivative of papain in which the free thiol group of the proteinase is attached to a poly(ethylene glycol) chain via a disulphide bond has been prepared. The poly(ethylene glycol) chain confers to the conjugate new physicochemical properties, including a looser binding to ion exchange chromatographic supports such as CM-Sephadex C-50. The conjugate is thus readily separated from the irreversibly oxidized (unconjugated) forms of the enzyme. A papain preparation titrating 0.97 mol of thiol per mol of enzyme is obtained from the conjugate.

引用

页码：1413 / 1417

页数：5

共 31 条

[1] NUCLEOTIDE-SEQUENCE OF CDNA FOR SULFHYDRYL-ENDOPEPTIDASE (SH-EP) FROM COTYLEDONS OF GERMINATING VIGNA-MUNGO SEEDS [J].

AKASOFU, H ;

YAMAUCHI, D ;

MITSUHASHI, W ;

MINAMIKAWA, T .

NUCLEIC ACIDS RESEARCH, 1989, 17 (16) :6733-6733

[2] STRUCTURE OF ACTINIDIN, AFTER REFINEMENT AT 1.7-A RESOLUTION [J].

BAKER, EN .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 141 (04) :441-484

[3] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX [J].

BROCKLEHURST, K ;

CARLSSON, J ;

KIERSTAN, MP ;

CROOK, EM .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :573-584

[4]

BRYGIER J, 1993, BIOTECHNOL APPL BIOC, V43, P127

[5]

CABIAUX V, 1989, J BIOL CHEM, V264, P4928

[6] AMINO-ACID SEQUENCE OF TRYPTIC PEPTIDES FROM ACTINIDIN, A PROTEOLYTIC-ENZYME FROM FRUIT OF ACTINIDIA-CHINESIS [J].

CARNE, A ;

MOORE, CH .

BIOCHEMICAL JOURNAL, 1978, 173 (01) :73-83

[7]

COHEN LW, 1986, GENE, V48, P219, DOI 10.1016/0378-1119(86)90080-6

[8] PURIFICATION AND CHARACTERIZATION OF THAUMATOPAIN, A CYSTEINE PROTEASE FROM THE ARILS OF THE PLANT THAUMATOCOCCUS-DANIELLII [J].

CUSACK, M ;

STEPHEN, AG ;

POWLS, R ;

BEYNON, RJ .

BIOCHEMICAL JOURNAL, 1991, 274 :231-236

[9]

DELGADO C, 1990, BIOTECHNOL APPL BIOC, V12, P119

[10] THE THIOL PROTEINASES FROM THE LATEX OF CARICA-PAPAYA L .2. THE PRIMARY STRUCTURE OF PROTEINASE-OMEGA [J].

DUBOIS, T ;

KLEINSCHMIDT, T ;

SCHNEK, AG ;

LOOZE, Y ;

BRAUNITZER, G .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1988, 369 (08) :741-754

← 1 2 3 4 →