DETECTION OF HEPATIC MICROMETASTASIS IN PANCREATIC ADENOCARCINOMA PATIENTS BY 2-STAGE POLYMERASE CHAIN-REACTION RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS

被引:57
作者
INOUE, S [1 ]
NAKAO, A [1 ]
KASAI, Y [1 ]
HARADA, A [1 ]
NONAMI, T [1 ]
TAKAGI, H [1 ]
机构
[1] NAGOYA UNIV, SCH MED, DEPT SURG 2, SHOWA KU, NAGOYA, AICHI 466, JAPAN
来源
JAPANESE JOURNAL OF CANCER RESEARCH | 1995年 / 86卷 / 07期
关键词
PANCREATIC CANCER; K-RAS; PCR/RFLP ANALYSIS; MICROMETASTASIS;
D O I
10.1111/j.1349-7006.1995.tb02444.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Hepatic metastasis and retroperitoneal recurrence generally are considered to be the two primary modes of recurrence in pancreatic cancer. The goal of this study was to determine if patients with pancreatic adenocarcinoma have hepatic and peritoneal micrometastasis at operation. Pancreatic adenocarcinomas are known to have a high incidence of K-ras gene mutations. Liver tissue specimens were obtained from 30 patients (17 with pancreatic adenocarcinoma and 13 with other diseases) with a biopsy needle at operation. Peritoneal washings were obtained during operation from 20 patients with pancreatic adenocarcinoma. Two-stage polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to detect K-ras oncogene mutation at codon 12. Thirteen of 17 pancreatic adenocarcinoma patients had K-ras gene mutations in the liver, whereas all 13 patients with other diseases did not. However, only two of 20 pancreatic adenocarcinoma patients revealed K-ras gene mutation in peritoneal lavage fluids. These results indicate the feasibility of detecting hepatic micrometastasis in patients with pancreatic adenocarcinoma, and imply that PCR/RFLP analysis may be of value in the diagnosis, treatment and follow-up of hepatic metastasis of pancreatic adenocarcinoma.
引用
收藏
页码:626 / 630
页数:5
相关论文
共 24 条
  • [1] MOST HUMAN CARCINOMAS OF THE EXOCRINE PANCREAS CONTAIN MUTANT C-K-RAS GENES
    ALMOGUERA, C
    SHIBATA, D
    FORRESTER, K
    MARTIN, J
    ARNHEIM, N
    PERUCHO, M
    [J]. CELL, 1988, 53 (04) : 549 - 554
  • [2] RAS GENE-MUTATIONS AND HPV INFECTION ARE COMMON IN HUMAN LARYNGEAL CARCINOMA
    ANWAR, K
    NAKAKUKI, K
    NAIKI, H
    INUZUKA, M
    [J]. INTERNATIONAL JOURNAL OF CANCER, 1993, 53 (01) : 22 - 28
  • [3] CALDAS C, 1994, CANCER RES, V54, P3568
  • [4] ACTIVATION OF KI-RAS 2 GENE IN HUMAN-COLON AND LUNG CARCINOMAS BY 2 DIFFERENT POINT MUTATIONS
    CAPON, DJ
    SEEBURG, PH
    MCGRATH, JP
    HAYFLICK, JS
    EDMAN, U
    LEVINSON, AD
    GOEDDEL, DV
    [J]. NATURE, 1983, 304 (5926) : 507 - 513
  • [6] HIGH-FREQUENCY OF KI-RAS CODON-12 MUTATIONS IN PANCREATIC ADENOCARCINOMAS
    GRUNEWALD, K
    LYONS, J
    FROHLICH, A
    FEICHTINGER, H
    WEGER, RA
    SCHWAB, G
    JANSSEN, JWG
    BARTRAM, CR
    [J]. INTERNATIONAL JOURNAL OF CANCER, 1989, 43 (06) : 1037 - 1041
  • [7] HAYASHI N, 1994, CANCER RES, V54, P3853
  • [8] KAHN SM, 1991, ONCOGENE, V6, P1079
  • [9] INFREQUENT RAS MUTATION IN HUMAN STOMACH CANCERS
    KOSHIBA, M
    OGAWA, O
    HABUCHI, T
    HAMAZAKI, S
    SHIMADA, T
    TAKAHASHI, R
    SUGIYAMA, T
    [J]. JAPANESE JOURNAL OF CANCER RESEARCH, 1993, 84 (02): : 163 - 167
  • [10] MUTATIONS OF KI-RAS ONCOGENE CODON-12 IN BETEL QUID CHEWING-RELATED HUMAN ORAL SQUAMOUS-CELL CARCINOMA IN TAIWAN
    KUO, MYP
    JENG, JH
    CHIANG, CP
    HAHN, LJ
    [J]. JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1994, 23 (02) : 70 - 74