INVITRO SYNTHESIS OF BACTERIOOPSIN - INTEGRATION INTO MICROSOMAL-MEMBRANES

被引:3
作者
BAUER, U
HILDEBRANDT, V
DENCHER, NA
WREDE, P
机构
[1] FREE UNIV BERLIN,FACHBEREICH PHYS,AKTIEUNG GESELL BIOPHYS,ARNIMALLEE 14,W-1000 BERLIN 33,GERMANY
[2] HAHN MEITNER INST KERNFORSCH BERLIN GMBH,BENSC N1,W-1000 BERLIN 39,GERMANY
关键词
D O I
10.1016/0006-291X(92)90469-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The translation and membrane integration of bacterio-opsin from Halobacterium salinarium were investigated. Plasmids containing the bacterio-opsin-gene with or without its original presequence were transcribed with the T7-RNA-polymerase and translated in vitro in a wheat germ system. The integration of the expressed bacterio-opsin into dog pancreas microsomes was studied. Both precursor bacterio-opsin and mature bacterio-opsin integrate into the eukaryotic membrane. © 1992.
引用
收藏
页码:1480 / 1485
页数:6
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