INVITRO SYNTHESIS OF BACTERIOOPSIN - INTEGRATION INTO MICROSOMAL-MEMBRANES

被引：3

作者：

BAUER, U

HILDEBRANDT, V

DENCHER, NA

WREDE, P

机构：

[1] FREE UNIV BERLIN,FACHBEREICH PHYS,AKTIEUNG GESELL BIOPHYS,ARNIMALLEE 14,W-1000 BERLIN 33,GERMANY

[2] HAHN MEITNER INST KERNFORSCH BERLIN GMBH,BENSC N1,W-1000 BERLIN 39,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)90469-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The translation and membrane integration of bacterio-opsin from Halobacterium salinarium were investigated. Plasmids containing the bacterio-opsin-gene with or without its original presequence were transcribed with the T7-RNA-polymerase and translated in vitro in a wheat germ system. The integration of the expressed bacterio-opsin into dog pancreas microsomes was studied. Both precursor bacterio-opsin and mature bacterio-opsin integrate into the eukaryotic membrane. © 1992.

引用

收藏

页码：1480 / 1485

页数：6

相关论文

共 22 条

[1] BACTERIORHODOPSIN - PARTIAL SEQUENCE OF MESSENGER-RNA PROVIDES AMINO-ACID-SEQUENCE IN THE PRECURSOR REGION [J].

CHANG, SH ;

MAJUMDAR, A ;

DUNN, R ;

MAKABE, O ;

RAJBHANDARY, UL ;

KHORANA, HG ;

OHTSUKA, E ;

TANAKA, T ;

TANIYAMA, YO ;

IKEHARA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3398-3402

[2] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[3] FORMATION OF A FUNCTIONAL RIBOSOME-MEMBRANE JUNCTION DURING TRANSLOCATION REQUIRES THE PARTICIPATION OF A GTP-BINDING PROTEIN [J].

CONNOLLY, T ;

GILMORE, R .

JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2253-2261

[4] BACTERIO-OPSIN MESSENGER-RNA IN WILD-TYPE AND BACTERIO-OPSIN-DEFICIENT HALOBACTERIUM-HALOBIUM STRAINS [J].

DASSARMA, S ;

RAJBHANDARY, UL ;

KHORANA, HG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (01) :125-129

[5]

DELLWEG HG, 1980, FEBS LETT, V116, P303, DOI 10.1016/0014-5793(80)80668-5

[6] THE BACTERIORHODOPSIN GENE [J].

DUNN, R ;

MCCOY, J ;

SIMSEK, M ;

MAJUMDAR, A ;

CHANG, SH ;

RAJBHANDARY, UL ;

KHORANA, HG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6744-6748

[7] TOPOGRAPHY OF SURFACE-EXPOSED AMINO-ACIDS IN THE MEMBRANE-PROTEIN BACTERIORHODOPSIN DETERMINED BY PROTEOLYSIS AND MICRO-SEQUENCING [J].

FIMMEL, S ;

CHOLI, T ;

DENCHER, NA ;

BULDT, G ;

WITTMANNLIEBOLD, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 978 (02) :231-240

[8] FULL-LENGTH PREPRO-ALPHA-FACTOR CAN BE TRANSLOCATED ACROSS THE MAMMALIAN MICROSOMAL MEMBRANE ONLY IF TRANSLATION HAS NOT TERMINATED [J].

GARCIA, PD ;

WALTER, P .

JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1043-1048

[9] INVITRO TRANSLATION OF HALOBACTERIAL MESSENGER-RNA [J].

GROPP, R ;

OESTERHELT, D .

FEBS LETTERS, 1989, 259 (01) :5-9

[10] ASSOCIATION OF THE HALOBACTERIAL-7S RNA TO THE POLYSOME CORRELATES WITH EXPRESSION OF THE MEMBRANE-PROTEIN BACTERIOOPSIN [J].

GROPP, R ;

GROPP, F ;

BETLACH, MC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (04) :1204-1208