LOCALIZATION OF THE MESSENGER-RNA FOR THE 5-HT2 RECEPTOR BY INSITU HYBRIDIZATION HISTOCHEMISTRY - CORRELATION WITH THE DISTRIBUTION OF RECEPTOR-SITES

被引：200

作者：

MENGOD, G

POMPEIANO, M

MARTINEZMIR, MI

PALACIOS, JM

机构：

[1] Preclinical Research, Sandoz Pharma Ltd.

来源：

BRAIN RESEARCH | 1990年 / 524卷 / 01期

关键词：

In situ hybridization; mRNA; Oligonucleotide; Rat brain; Receptor autoradiography; Serotonin-2; receptor;

D O I：

10.1016/0006-8993(90)90502-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

32P-labelled oligonucleotides complementary to rat 5-HT2 receptor mRNA were used as probes to study the distribution of cells in rat brain containing the mRNA coding for this receptor by in situ hybridization histochemistry. 5-HT2 receptor binding sites were visualized by autoradiography using [125I]DOI as ligand. Both distributions were comparable, demonstrating that 5-HT2 receptors are expressed by cells intrinsic to the neocortex (lamina Va), claustrum, olfactory bulb and several nuclei of the brainstem. © 1990.

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页码：139 / 143

页数：5

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