EFFECTS OF 5,8,11,14-EICOSATETRAYNOIC ACID ON THAPSIGARGIN-INDUCED CALCIUM-ENTRY, AND INTRACELLULAR PH IN THYROID FRTL-5 CELLS

被引：7

作者：

EKOKOSKI, E

TORNQUIST, K

机构：

[1] MINERVA FDN, INST MED RES, SF-00250 HELSINKI, FINLAND

[2] UNIV HELSINKI, DEPT ZOOL, DIV PHYSIOL, SF-00100 HELSINKI, FINLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1223卷 / 02期

基金：

芬兰科学院;

关键词：

ENZYME INHIBITION; LIPOXYGENASE; CYTOCHROME P-450; CALCIUM INFLUX;

D O I：

10.1016/0167-4889(94)90237-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of 5,8,11,14-eicosatetraynoic acid (ETYA), an inhibitor of lipoxygenase and cytochrome P-450 epoxygenase enzymes, on calcium fluxes was investigated in Fura 2 loaded rat thyroid FRTL-5 cells. ETYA per se released sequestered calcium. ETYA also inhibited calcium influx in thapsigargin-stimulated cells in a dose-dependent manner. Addition of calcium to cells treated with ETYA and stimulated with thapsigargin in a calcium-free buffer resulted in a blunted increase in intracellular free calcium compared with the response in control cells. In addition, ETYA per se acidified the cytosol in a dose-dependent manner. Acidification of the cytosol with the K+/H+ ionophore nigericin also decreased thapsigargin-induced calcium entry, but not to the same extent as that seen in cells treated with ETYA. The results suggest that ETYA is a patent modulator of calcium entry, and that part of the inhibitory effect of ETYA may be due to the ETYA-induced acidification of the cytosol.

引用

页码：274 / 278

页数：5

共 29 条

[11]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[12] EFFECTS OF PH, REDUCING AND ALKYLATING REAGENTS ON THE BINDING AND CA-2+ RELEASE ACTIVITIES OF INOSITOL 1,4,5-TRIPHOSPHATE IN THE BOVINE ADRENAL-CORTEX [J].