IMMOBILIZATION OF PERMEABILIZED ESCHERICHIA-COLI-CELLS WITH PENICILLIN ACYLASE ACTIVITY

被引：29

作者：

PRABHUNE, AA ^{[1
]}

RAO, BS ^{[1
]}

PUNDLE, AV ^{[1
]}

SIVARAMAN, H ^{[1
]}

机构：

[1] NATL CHEM LAB, DIV BIOCHEM SCI, POONA 411008, MAHARASHTRA, INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 02期

关键词：

ESCHERICHIA-COLI; PENICILLIN ACYLASE; WHOLE CELLS; PERMEABILIZATION; IMMOBILIZATION; POLYACRYLAMIDE GEL;

D O I：

10.1016/0141-0229(92)90176-O

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli cells with penicillin acylase activity were sequentially treated at pH 7.8 with aqueous solutions of N-cetyl-N,N,N-trimethylammonium bromide and glutaraldehyde and then immobilized within porous polyacrylamide beads. The immobilized whole cells showed enhanced hydrolysis rates in the conversion of benzylpenicillin to 6-aminopenicillanic acid (6-APA) compared to untreated cells immobilized and used under identical conditions. The immobilized system showed no apparent loss in enzyme activity when used repeatedly over 90 cycles for 6-APA production from 4% benzylpenicillin.

引用

页码：161 / 163

页数：3

共 13 条

[1] ISOLATION AND KINETICS OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI [J].

BALASINGHAM, K ;

WARBURTON, D ;

DUNNILL, P ;

LILLY, MD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 276 (01) :250-+

[2] THE 2 SUBUNITS OF PENICILLIN ACYLASE ARE PROCESSED FROM A COMMON PRECURSOR [J].

BOCK, A ;

WIRTH, R ;

SCHMID, G ;

SCHUMACHER, G ;

LANG, G ;

BUCKEL, P .

FEMS MICROBIOLOGY LETTERS, 1983, 20 (02) :141-144

[3] AUTOMATED COLORIMETRIC DETERMINATION OF 6-AMINOPENICILLANIC ACID IN FERMENTATION MEDIA [J].

BOMSTEIN, J ;

EVANS, WG .

ANALYTICAL CHEMISTRY, 1965, 37 (04) :576-&

[4] PERMEABILIZED CELLS [J].

FELIX, H .

ANALYTICAL BIOCHEMISTRY, 1982, 120 (02) :211-234

[5] PERMEABILIZATION OF YEAST-CELLS (KLUYVEROMYCES-FRAGILIS) TO LACTOSE BY CETYLTRIMETHYLAMMONIUM BROMIDE [J].

JOSHI, MS ;

GOWDA, LR ;

BHAT, SG .

BIOTECHNOLOGY LETTERS, 1987, 9 (08) :549-554

[6] THE PRODUCTION OF 6-AMINOPENICILLANIC ACID BY A MULTISTAGE TUBULAR REACTOR PACKED WITH IMMOBILIZED PENICILLIN AMIDASE [J].

PARK, JM ;

CHOI, CY ;

SEONG, BL ;

HAN, MH .

BIOTECHNOLOGY AND BIOENGINEERING, 1982, 24 (07) :1623-1637

[7] EVIDENCE FOR INVOLVEMENT OF ARGINYL RESIDUE AT THE CATALYTIC SITE OF PENICILLIN ACYLASE FROM ESCHERICHIA-COLI [J].

PRABHUNE, AA ;

SIVARAMAN, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) :317-322

[8]

PRABHUNE AA, 1991, IN PRESS APPL BIOCH

[9] IMMOBILIZATION OF SACCHAROMYCES-UVARUM CELLS IN POROUS BEADS OF POLYACRYLAMIDE-GEL FOR ETHANOLIC FERMENTATION [J].

PUNDLE, A ;

PRABHUNE, A ;

SIVARAMAN, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 29 (05) :426-429

[10] CONTINUOUS PRODUCTION OF 6-AMINOPENICILLANIC ACID FROM PENICILLIN BY IMMOBILIZED MICROBIAL-CELLS [J].

SATO, T ;

TOSA, T ;

CHIBATA, I .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY, 1976, 2 (03) :153-160

← 1 2 →