DEMONSTRATION OF A HEPATITIS-C VIRUS-SPECIFIC ANTIGEN PREDICTED FROM THE PUTATIVE CORE GENE IN THE CIRCULATION OF INFECTED HOSTS

被引:47
作者
TAKAHASHI, K
OKAMOTO, H
KISHIMOTO, S
MUNEKATA, E
TACHIBANA, K
AKAHANE, Y
YOSHIZAWA, H
MISHIRO, S
机构
[1] INST IMMUNOL, KORAKU 1-1-10, BUNKYO KU, TOKYO 112, JAPAN
[2] HAMAMATSU UNIV SCH MED, DEPT PUBL HLTH, HAMAMATSU, SHIZUOKA 431-31, JAPAN
[3] JICHI MED SCH, DIV IMMUNOL, MINAMI KAWACHI, TOCHIGI 32904, JAPAN
[4] UNIV TSUKUBA, DEPT APPL BIOCHEM, TSUKUBA, IBARAKI 305, JAPAN
[5] JAPANESE RED CROSS BLOOD CTR, SAITAMA 388, JAPAN
[6] YAMANASHI MED COLL, DEPT INTERNAL MED 1, YAMANASHI 42938, JAPAN
[7] HIROSHIMA UNIV, SCH MED, DEPT HYG, HIROSHIMA 734, JAPAN
关键词
D O I
10.1099/0022-1317-73-3-667
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An ELISA was used to detect a protein derived from the core gene of the hepatitis C virus (HCV) in human plasma. The solid phase antibody in the assay was a murine monoclonal antibody against a synthetic peptide deduced from the putative core gene of HCV (residues 39 to 74). An enzyme-labelled affinity-purified human antibody directed at another region within the HCV core (residues 5 to 23) was the second antibody tracer. The ELISA had a sensitivity capable of detecting a few ng/ml of the HCV core polypeptide expressed in Escherichia coli. Core antigen activity in plasma of infected hosts was detected after treatment of HCV RNA-rich fractions from buoyant density centrifugation with the detergent Tween 80. There was a direct correlation between core antigen ELISA values of a plasma fraction and intensities of polymerase chain reaction signals for HCV RNA. These observations are consistent with the proposal that the N-terminal sequence of the predicted polyprotein of HCV is a nucleocapsid protein, and that improved core antigen assays may correlate with viraemia.
引用
收藏
页码:667 / 672
页数:6
相关论文
共 18 条
  • [1] ALMEIDA JD, 1971, LANCET, V2, P1225
  • [2] POST-TRANSFUSION NON-A, NON-B HEPATITIS - PHYSICOCHEMICAL PROPERTIES OF 2 DISTINCT AGENTS
    BRADLEY, DW
    MAYNARD, JE
    POPPER, H
    COOK, EH
    EBERT, JW
    MCCAUSTLAND, KA
    SCHABLE, CA
    FIELDS, HA
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1983, 148 (02) : 254 - 265
  • [3] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME
    CHOO, QL
    KUO, G
    WEINER, AJ
    OVERBY, LR
    BRADLEY, DW
    HOUGHTON, M
    [J]. SCIENCE, 1989, 244 (4902) : 359 - 362
  • [4] GENETIC ORGANIZATION AND DIVERSITY OF THE HEPATITIS-C VIRUS
    CHOO, QL
    RICHMAN, KH
    HAN, JH
    BERGER, K
    LEE, C
    DONG, C
    GALLEGOS, C
    COIT, D
    MEDINASELBY, A
    BARR, PJ
    WEINER, AJ
    BRADLEY, DW
    KUO, G
    HOUGHTON, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) : 2451 - 2455
  • [5] DANE DS, 1970, LANCET, V1, P695
  • [6] HOFFNAGLE JH, 1973, LANCET, V2, P869
  • [7] MOLECULAR-CLONING OF THE HUMAN HEPATITIS-C VIRUS GENOME FROM JAPANESE PATIENTS WITH NON-A, NON-B HEPATITIS
    KATO, N
    HIJIKATA, M
    OOTSUYAMA, Y
    NAKAGAWA, M
    OHKOSHI, S
    SUGIMURA, T
    SHIMOTOHNO, K
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) : 9524 - 9528
  • [8] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS
    KUO, G
    CHOO, QL
    ALTER, HJ
    GITNICK, GL
    REDEKER, AG
    PURCELL, RH
    MIYAMURA, T
    DIENSTAG, JL
    ALTER, MJ
    STEVENS, CE
    TEGTMEIER, GE
    BONINO, F
    COLOMBO, M
    LEE, WS
    KUO, C
    BERGER, K
    SHUSTER, JR
    OVERBY, LR
    BRADLEY, DW
    HOUGHTON, M
    [J]. SCIENCE, 1989, 244 (4902) : 362 - 364
  • [10] EXTRAORDINARILY LOW-DENSITY OF HEPATITIS-C VIRUS ESTIMATED BY SUCROSE DENSITY GRADIENT CENTRIFUGATION AND THE POLYMERASE CHAIN-REACTION
    MIYAMOTO, H
    OKAMOTO, H
    SATO, K
    TANAKA, T
    MISHIRO, S
    [J]. JOURNAL OF GENERAL VIROLOGY, 1992, 73 : 715 - 718