RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) NEUROPEPTIDE-Y

被引：12

作者：

BARTON, CL

SHAW, C

HALTON, DW

THIM, L

机构：

[1] QUEENS UNIV BELFAST, CLIN MED, BELFAST BT7 1NN, ANTRIM, NORTH IRELAND

[2] QUEENS UNIV BELFAST, SCH BIOL & BIOCHEM, COMPARAT NEUROENDOCRINOL RES GRP, BELFAST BT7 1NN, ANTRIM, NORTH IRELAND

[3] NOVO NORDISK AS, BAGSVAERD, DENMARK

来源：

PEPTIDES | 1992年 / 13卷 / 06期

关键词：

RAINBOW TROUT; NEUROPEPTIDE-Y; COHO SALMON; PANCREATIC POLYPEPTIDE; GAS-PHASE SEQUENCING; MASS SPECTROSCOPY; PRIMARY STRUCTURE; PEPTIDE PHYLOGENY;

D O I：

10.1016/0196-9781(92)90023-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuropeptide Y (NPY) has been isolated from brain extracts of the rainbow trout (Oncorhynchus mykiss) and subjected to structural analyses. Plasma desorption mass spectroscopy estimated the molecular mass of the purified peptide as 4303.9 Da. Automated Edman degradation unequivocally established the sequence of a 36 amino acid residue peptide as: Tyr-Pro-Pro-Lys-Pro-Glu-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Pro-Glu-Glu-Leu-Ala-Lys-T yr-Tyr-Thr-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr. The molecular mass calculated from this sequence (4304 Da) is consistent with that obtained by mass spectroscopy. The presence of a C-terminal amide was established by radioimmunoassay. Rainbow trout NPY is identical in primary structure to coho salmon (Oncorhynchus kisutch) pancreatic polypeptide (PP). These data may indicate that, in this group of salmonid fishes, a single member of the NPY/PP peptide family is expressed in both neurons and peripheral endocrine cells.

引用

页码：1159 / 1163

页数：5

共 31 条

[21] LOCALIZATION, QUANTIFICATION, AND CHARACTERIZATION OF PANCREATIC-POLYPEPTIDE IMMUNOREACTIVITY IN THE PARASITIC FLATWORM DICLIDOPHORA-MERLANGI AND ITS FISH HOST (MERLANGIUS-MERLANGUS)
MAULE, AG
SHAW, C
HALTON, DW
JOHNSTON, CF
FAIRWEATHER, I
[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1989, 74 (01) : 50 - 56
[22] NEUROPEPTIDE-F - A NOVEL PARASITIC FLATWORM REGULATORY PEPTIDE FROM MONIEZIA-EXPANSA (CESTODA, CYCLOPHYLLIDEA)
MAULE, AG
SHAW, C
HALTON, DW
THIM, L
JOHNSTON, CF
FAIRWEATHER, I
BUCHANAN, KD
[J]. PARASITOLOGY, 1991, 102 : 309 - 316
[23] THE COMPLETE PRIMARY STRUCTURE OF PANCREATIC-POLYPEPTIDE FROM THE EUROPEAN COMMON FROG, RANA-TEMPORARIA
MCKAY, DM
SHAW, C
THIM, L
JOHNSTON, CF
HALTON, DW
FAIRWEATHER, I
BUCHANAN, KD
[J]. REGULATORY PEPTIDES, 1990, 31 (03) : 187 - 198
[24] THE PRIMARY STRUCTURE AND TISSUE DISTRIBUTION OF AN AMPHIBIAN NEUROPEPTIDE-Y
MCKAY, DM
SHAW, C
HALTON, DW
THIM, L
BUCHANAN, KD
[J]. REGULATORY PEPTIDES, 1992, 37 (02) : 143 - 153
[25] OHARE MMT, 1989, CANCER RES, V49, P7010
[26] THE PRIMARY STRUCTURE OF PEPTIDE-Y (PY) OF THE SPINY DOGFISH, SQUALUS-ACANTHIAS - IMMUNOCYTOCHEMICAL LOCALIZATION AND ISOLATION FROM THE PANCREAS
PAN, JZ
SHAW, C
HALTON, DW
THIM, L
JOHNSTON, CF
BUCHANAN, KD
[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1992, 102 (01): : 1 - 5
[27] POLLOCK HG, 1988, J BIOL CHEM, V263, P9746
[28] POLLOCK HG, 1987, GEN COMP ENDOCR, V61, P375
[29] NEURONAL LOCALIZATION OF PANCREATIC POLYPEPTIDE (PP) AND VASOACTIVE INTESTINAL PEPTIDE (VIP) IMMUNOREACTIVITY IN EARTHWORM (LUMBRICUS-TERRESTRIS)
SUNDLER, F
HAKANSON, R
ALUMETS, J
WALLES, B
[J]. BRAIN RESEARCH BULLETIN, 1977, 2 (01) : 61 - 65
[30] ISOLATION AND CHARACTERIZATION OF PEPTIDE YY (PYY), A CANDIDATE GUT HORMONE THAT INHIBITS PANCREATIC EXOCRINE SECRETION
TATEMOTO, K
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (08): : 2514 - 2518

← 1 2 3 4 →