POLY 2'-O-METHYLCYTIDYLIC ACID AND ROLE OF 2'-HYDROXYL IN POLYNUCLEOTIDE STRUCTURE

被引：51

作者：

ZMUDZKA, B

JANION, C

SHUGAR, D

机构：

[1] Department of Radiobiology, Institute of Oncology University of Warsaw, Warsaw

[2] Institute of Biochemistry and Biophysics, Academy of Sciences University of Warsaw, Warsaw

[3] Department of Biophysics, University of Warsaw, Warsaw

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 06期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0006-291X(69)90215-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the presence of Mn++ cations, 2′-O-methylcytidine-5′-pyrophosphate is readily polymerized by polynucleotide phosphorylase. The neutral form of the resulting poly 2′-O-methylcytidylic acid more closely resembles the neutral, single-stranded form of poly rC than poly dC. The polymer forms a twin-stranded helix in the same pH range as poly rC, but not poly dC, showing that formation of the acid form of poly rC does not involve hydrogen bonding of the 2′-OH to a base residue or phosphate oxygen. Poly 2′-O-MeC also forms a double-stranded helix with poly rI. Implications as to the role of the 2′-OH in polynucleotide structure are briefly discussed. © 1969.

引用

页码：895 / &

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[21]

SWIERKOWSKI M, IN PRESS

[22] STRUCTURE OF POLY-5-METHYLCYTIDYLIC ACID AND ITS TWIN-STRANDED COMPLEX WITH POLY-INOSINIC ACID [J].