ENDOTOXIC GLYCOLIPID AS A POTENT DEPRESSOR OF THE HEPATIC-METABOLIZING ENZYME-SYSTEMS IN MICE

Inhibitory effects of the endotoxic glycolipid from Salmonella minnesota R595 on hepatic drug‐metabolizing enzyme activities in mice were investigated, and the depressor activity of the glycolipid in the enzyme systems was confirmed. Among degradation products of lipopolysaccharides tested, lipid A preparations derived from the mild acetic acid hydrolysates of lipopolysaccharides were the most active, but the lipid A fractions prepared from the hydrolysates with 1 N‐HCl were almost inactive. A degraded polysaccharide fraction from E. coli lipopolysaccharide was inactive. The activities of the glycolipid and the lipid A preparation were markedly reduced by treatment with alkaline‐hydroxylamine, mild alkali or hydrazine. The data showed that the lipid A moiety of the glycolipid may be responsible for the inhibitory activity on the hepatic drug‐metabolizing enzyme systems. © owned by Center for Academic Publications Japan (Publisher)