INTRAMITOCHONDRIAL K+ AS ACTIVATOR OF CARBOXYATRACTYLOSIDE-INDUCED CA-2+ RELEASE

被引:31
作者
CHAVEZ, E
MORENOSANCHEZ, R
ZAZUETA, C
REYESVIVAS, H
ARTEAGA, D
机构
[1] Departamento de Bioquímica, Instituto Nacional de Cardiologia, Ignacio Chavez, Mexico, D.F.
关键词
MEMBRANE PERMEABILITY; MITOCHONDRIAL MEMBRANE; CARBOXYATRACTYLOSIDE; CALCIUM RELEASE;
D O I
10.1016/0005-2736(91)90087-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of intramitochondrial K+ content on the increase in membrane permeability to Ca2+, as induced by carboxyatractyloside was studied. In mitochondria containing a high K+ concentration (83 nmol/mg), carboxyatractyloside induced a fast and extensive mitochondrial Ca2+ release, membrane de-energization, and swelling. Conversely, in K+-depleted mitochondria (11 nmol/mg), carboxyatractyloside was ineffective. The addition of 40 mM K+ to K+-depleted mitochondria restored the capability of atractyloside to induce an increase in membrane permeability to Ca2+ release. The determination of matrix free Ca2+ concentration showed that, at an external free-Ca2+ concentration of 0.8-mu-M, control mitochondria contained 3.9-mu-M of free Ca2+ whereas K+-depleted mitochondria contained 0.9-mu-M free Ca2+. It is proposed that intramitochondrial K+ affects the matrix free Ca2+ concentration required to induce a state of high membrane permeability.
引用
收藏
页码:461 / 466
页数:6
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