A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
[8]
Grula J. W., 1987, Plant gene systems and their biology. Proceedings of a CIBA-Geigy-UCLA symposium held at Tamarron, Colorado, USA, 2-8 February, 1987, P207
[8]
Grula J. W., 1987, Plant gene systems and their biology. Proceedings of a CIBA-Geigy-UCLA symposium held at Tamarron, Colorado, USA, 2-8 February, 1987, P207