CLONING AND SEQUENCING OF THE TUF GENES OF STREPTOMYCES-COELICOLOR A3(2)

被引：20

作者：

VANWEZEL, GP

WOUDT, LP

VERVENNE, R

VERDURMEN, MLA

VIJGENBOOM, E

BOSCH, L

机构：

[1] Leiden University, Gorlaeus Laboratories, 2300 RA Leiden

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1219卷 / 02期

关键词：

ELONGATION FACTOR TU; SEQUENCE COMPARISON; GENE CLONING; (STREPTOMYCES);

D O I：

10.1016/0167-4781(94)90085-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two tuf genes are present in Streptomyces coelicolor A3(2), which have been cloned and sequenced. These genes show a high degree of nucleotide sequence identity to the tuf1 and tuf3 genes of Streptomyces ramocissimus: the tuf1 genes are 94% identical, the tuf3 genes 87%. S. coelicolor tuf1 encodes a protein of 396 amino acids, while tuf3 encodes a protein of 391 amino acids.

引用

收藏

页码：543 / 547

页数：5

相关论文

共 13 条

[1] THE NUCLEOTIDE-SEQUENCE OF TUFB AND 4 NEARBY TRANSFER-RNA STRUCTURAL GENES OF ESCHERICHIA-COLI [J].

AN, G ;

FRIESEN, JD .

GENE, 1980, 12 (1-2) :33-39

[2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .6. PLASMID PBR329, A NEW DERIVATIVE OF PBR328 LACKING THE 482-BASE-PAIR INVERTED DUPLICATION [J].

COVARRUBIAS, L ;

BOLIVAR, F .

GENE, 1982, 17 (01) :79-89

[3] GTP-BINDING DOMAIN - 3 CONSENSUS SEQUENCE ELEMENTS WITH DISTINCT SPACING [J].

DEVER, TE ;

GLYNIAS, MJ ;

MERRICK, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1814-1818

[4] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[5] DUPLICATION OF THE TUF GENE, WHICH ENCODES PEPTIDE-CHAIN ELONGATION-FACTOR TU, IS WIDESPREAD IN GRAM-NEGATIVE BACTERIA [J].

FILER, D ;

FURANO, AV .

JOURNAL OF BACTERIOLOGY, 1981, 148 (03) :1006-1011

[6]

LINDAHL L, 1986, ANNU REV GENET, V20, P297

[7] THE P-LOOP - A COMMON MOTIF IN ATP-BINDING AND GTP-BINDING PROTEINS [J].

SARASTE, M ;

SIBBALD, PR ;

WITTINGHOFER, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (11) :430-434

[8] DUPLICATION OF THE TUF GENE - A NEW INSIGHT INTO THE PHYLOGENY OF EUBACTERIA [J].

SELA, S ;

YOGEV, D ;

RAZIN, S ;

BERCOVIER, H .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :581-584

[9] TRANS-COMPLEMENTABLE COPY-NUMBER MUTANTS OF PLASMID COLE1 [J].

TWIGG, AJ ;

SHERRATT, D .

NATURE, 1980, 283 (5743) :216-218

[10]

VANDERMEIDE PH, 1983, EUR J BIOCHEM, V130, P397