TETRATHIAFULVALENE-MEDIATED BIOSENSOR FOR L-LACTATE IN DAIRY-PRODUCTS

被引：13

作者：

MULCHANDANI, A

BASSI, AS

NGUYEN, A

机构：

[1] Dept. of Chemical Engineering, Univ. of California, Riverside, California

[2] Dept. of Chemical & Biochemical Engineering, Univ. of Western Ontario, London, Ontario

来源：

JOURNAL OF FOOD SCIENCE | 1995年 / 60卷 / 01期

关键词：

TETRATHIAFULVALENE; LACTATE OXIDASE; BIOSENSOR; L-LACTATE; DAIRY PRODUCTS;

D O I：

10.1111/j.1365-2621.1995.tb05610.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A tetrathiafulvalene (TTF)-mediated, amperometric biosensor was developed for the specific determination of L-lactate. L-lactate oxidase was immobilized by adsorption and retained on the surface of a carbon-paste electrode using an electro-polymerized film of 1,3-phenylenediamineresorcinol. The response of the biosensor was maximum at pH 7.0 and 30 degrees C. Response was linear up to 0.6 mM lactate and had a minimum detection limit of 56 mu M. Response time was less than 90 sec. When stored at 4 degrees C response was stable for > 36 days and subsequently decreased. The sensor was unresponsive after 96 days. This enzyme-based biosensor was suitable for determining lactate concentrations in milk and yogurt.

引用

页码：74 / 78

页数：5

共 24 条

[1] A MEDIATED AMPEROMETRIC ENZYME ELECTRODE USING TETRATHIAFULVALENE AND L-GLUTAMATE OXIDASE FOR THE DETERMINATION OF L-GLUTAMIC ACID
ALMEIDA, NF
MULCHANDANI, AK
[J]. ANALYTICA CHIMICA ACTA, 1993, 282 (02) : 353 - 361
[2] Barker SB, 1941, J BIOL CHEM, V138, P535
[3] BERGMEYER HU, 1983, METHODS ENZYMATIC AN, V1
[4] REAGENTLESS ENZYME ELECTRODES FOR ETHANOL, LACTATE, AND MALATE
BLAEDEL, WJ
ENGSTROM, RC
[J]. ANALYTICAL CHEMISTRY, 1980, 52 (11) : 1691 - 1697
[5] BURINI G, 1993, J AOAC INT, V76, P1017
[6] SIMPLIFIED GAS-CHROMATOGRAPHIC PROCEDURE FOR IDENTIFICATION OF BACTERIAL METABOLIC PRODUCTS
CARLSSON, J
[J]. APPLIED MICROBIOLOGY, 1973, 25 (02) : 287 - 289
[7] FERROCENE-MEDIATED ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE
CASS, AEG
DAVIS, G
FRANCIS, GD
HILL, HAO
ASTON, WJ
HIGGINS, IJ
PLOTKIN, EV
SCOTT, LDL
TURNER, APF
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (04) : 667 - 671
[8] ELECTROPOLYMERIZED O-PHENYLENEDIAMINE FILM AS MEANS OF IMMOBILISING LACTATE OXIDASE FOR A L-LACTATE BIOSENSOR
DEMPSEY, E
WANG, J
SMYTH, MR
[J]. TALANTA, 1993, 40 (03) : 445 - 451
[9] CARBON PASTE-TETRATHIAFULVALENE AMPEROMETRIC ENZYME ELECTRODE FOR THE DETERMINATION OF GLUCOSE IN FLOWING SYSTEMS
GUNASINGHAM, H
TAN, CH
[J]. ANALYST, 1990, 115 (01) : 35 - 39
[10] AMPEROMETRIC GLUCOSE BIOSENSORS BASED ON REDOX POLYMER-MEDIATED ELECTRON-TRANSFER
HALE, PD
BOGUSLAVSKY, LI
INAGAKI, T
KARAN, HI
LEE, HS
SKOTHEIM, TA
OKAMOTO, Y
[J]. ANALYTICAL CHEMISTRY, 1991, 63 (07) : 677 - 682

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