SIGNIFICANCE OF EARLY INTRAALVEOLAR FIBROTIC LESIONS AND INTEGRIN EXPRESSION IN LUNG-BIOPSY SPECIMENS FROM PATIENTS WITH IDIOPATHIC PULMONARY FIBROSIS

被引：67

作者：

FUKUDA, Y

BASSET, F

FERRANS, VJ

YAMANAKA, N

机构：

[1] FAC XAVIER BICHAT,INSERM,U82,F-75018 PARIS,FRANCE

[2] NHLBI,PATHOL BRANCH,BETHESDA,MD 20892

来源：

HUMAN PATHOLOGY | 1995年 / 26卷 / 01期

关键词：

IDIOPATHIC PULMONARY FIBROSIS; INTEGRIN; ALPHA-5-BETA-1; VINCULIN; INTRAALVEOLAR FIBROSIS; CYTOPLASMIC HYALINE;

D O I：

10.1016/0046-8177(95)90114-0

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

To study the pulmonary structural remodeling in idiopathic pulmonary fibrosis (IPF), ultrastructural, immunohistochemical, and light microscopic molphometric observations were made on 11 pulmonary biopsy specimens from patients with IPF. The morphometric study was done using sequentially cut tissue sections stained for keratin-alcian blue periodic acid-Schiff (PAS), fibronectin, and type IV collagen-alcian blue PAS. Most of the early fibrotic lesions, which were alcian blue- and fibronectin-positive, were intra-alveolar in location. Intra-alveolar fibrosis is considered to be essential for the fusion of alveolar walls in IPF. A strong reaction for integrin alpha 5 beta 1 and vinculin was found in epithelial cells and mesenchymal cells in areas of intra-alveolar fibrosis. These finding show that these cells are active in adhesion to fibronectin in areas of early intra-alveolar fibrosis. Some of the epithelial cells, including cytoplasmic hyaline-laden cells, showed evidence of inadequate adhesion to the extracellular matrix, and this may constitute one of the mechanisms of progression of fibrosis in IPF. HUM PATHOL 26:53-61. Copyright (C) 1995 by W.B. Saunders Company

引用

页码：53 / 61

页数：9

共 29 条

[11]

Roman, McDonald, Expression of fibronectin, the integrin α5, and α-smooth muscle actin in heart and lung development, Am J Respir Cell Mol Biol, 6, pp. 472-480, (1992)

[12]

Chen, Chen, Mueller, Coupled expression and colocalization of 140K cell adhesion molecules fibronectin and laminin during morphogenesis and cytodifferentiation of chick lung cells, The Journal of Cell Biology, 103, pp. 1073-1090, (1986)

[13]

Albelda, Endothelial and epithelial cell adhesion molecules, Am J Respir Cell Mol Biol, 4, pp. 195-203, (1991)

[14]

Muschler, Horwitz, Down-regulation of the chicken α 5 β 1 integrin fibronectin receptor during development, Development, 113, pp. 327-337, (1991)

[15]

Geiger, Volk, Volberg, Et al., Molecular interactions in adherens-type contacts, J Cell Sci, 8, pp. 251-272, (1987)

[16]

Green, Geiger, Jones, Et al., The relationship between intermediate filaments and microfilaments before and during the formation of desmosomes and adherens-type junctions on mouse epidermal keratinocytes, 104, pp. 1389-1402, (1987)

[17]

Crystal, Bitterman, Rennard, Et al., Interstitial lung diseases of unknown cause, Disorders characterized by chronic inflammation of the lower respiratory tract, 310, pp. 154-166, (1984)

[18]

Crystal, Bitterman, Rennard, Et al., Interstitial lung diseases of unknown cause, Disorders characterized by chronic inflammation of the lower respiratory tract, 310, pp. 235-244, (1984)

[19]

Kino, Adachi, Yoshida, Et al., Characterization of a monoclonal antibody against human placenta type IV collagen by immunoelectroblotting, antibody-coupled affinity chromatography, and immunohistochemical localization, J Biochem, 103, pp. 829-835, (1988)

[20]

Kajikawa, Yamaguchi, Katsuda, Et al., An improved electron stain for elastic fibers using tannic acid, J Electron Microsc (Tokyo), 24, pp. 287-289, (1975)

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