EXPRESSION OF HUMAN LYSOSOMAL BETA-HEXOSAMINIDASE IN YEAST VACUOLES

被引：5

作者：

PREZANT, TR

机构：

[1] Department of Biological Chemistry, UCLA School of Medicine, Los Angeles

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91285-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The yeast Saccharomyces cerevisiae was tested as a recipient for functional expression of a mammalian lysosomal enzyme. The β chain of human β-hexosaminidase formed active dimeric enzyme, HexB, in transformants. HexB activity was localized to the vacuole, the yeast counterpart to the lysosome. A simple in situ enzyme assay was developed, which could be useful in expressing other lysosomal cDNAs. © 1990.

引用

页码：383 / 390

页数：8

共 31 条

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