THE SMALL HEAT-SHOCK PROTEIN HSP25 IS ACCUMULATED IN P19 EMBRYONAL CARCINOMA-CELLS AND EMBRYONIC STEM-CELLS OF LINE BLC6 DURING DIFFERENTIATION

被引：48

作者：

STAHL, J

WOBUS, AM

IHRIG, S

LUTSCH, G

BIELKA, H

机构：

[1] MAX DELBRUCK CTR MOLEC MED,ROBERT ROSSLE STR 10,W-1115 BERLIN,GERMANY

[2] INST PLANT GENET & CROP PLANT RES,W-4325 GATERSLEBEN,GERMANY

来源：

DIFFERENTIATION | 1992年 / 51卷 / 01期

关键词：

D O I：

10.1111/j.1432-0436.1992.tb00677.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Murine embryonal carcinoma and embryonic stem cell lines were investigated with regard to the occurrence of the small heat shock protein hsp25 during cell growth and differentiation. In the embryonal carcinoma cell line F9 considerable constitutive levels of hsp25 were observed which could be slightly increased by treatment with retinoic acid. No hsp25 was found, however, in the embryonal carcinoma cell line PCC4. When analyzing the pluripotent embryonal carcinoma cell line P19 and the pluripotent embryonic stem cell line BLC6, both characterized by high differentiation capacity, no hsp25 was observed under cell culture conditions maintaining the undifferentiated state. Induction of differentiation caused by prolonged cell culture, retinoic acid treatment, or embryoid body formation, however, resulted in an increase of the level of hsp25. The finding that hsp25 is accumulated in a differentiation-dependent manner suggests that this protein is associated with processes involved in differentiation. Therefore, hsp25 can be regarded as a marker of differentiation in the investigated embryonal carcinoma cell line P19 and the embryonic stem cell line BLC6.

引用

页码：33 / 37

页数：5

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