ENZYME ELECTRODE FOR THE SUCCESSIVE DETECTION OF HYPOXANTHINE AND INOSINE

被引：26

作者：

YAO, T ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE,COLL ENGN,DEPT APPL CHEM,SAKAI,OSAKA 593,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 281卷 / 02期

关键词：

AMPEROMETRY; BIOSENSORS; ENZYMATIC METHODS; ENZYME ELECTRODES; FISH; HYPOXANTHINE; INOSINE;

D O I：

10.1016/0003-2670(93)85188-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme electrode for the successive detection of hypoxanthine and inosine was constructed by co-immobilizing nucleoside phosphorylase and xanthine oxidase on a Nafion-coated platinum disc electrode. The successive responses for hypoxanthine and inosine were obtained in 0.1 M borate buffer (pH 7.5) without and with phosphate, respectively. The calibration graphs for hypoxanthine and inosine were linear up to 2 x 10(-4) M. The sensor could be used repeatedly for at least 20 days without deterioration of the response.

引用

页码：323 / 326

页数：4

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