ENTRY OF COXSACKIEVIRUS A9 INTO HOST-CELLS - SPECIFIC INTERACTIONS WITH ALPHA(V)BETA(3) INTEGRIN, THE VITRONECTIN RECEPTOR

被引:168
作者
ROIVAINEN, M
PIIRAINEN, L
HOVI, T
VIRTANEN, I
RIIKONEN, T
HEINO, J
HYYPIA, T
机构
[1] NATL PUBL HLTH INST,PROGRAM MOLEC BIOL,SF-00300 HELSINKI,FINLAND
[2] UNIV HELSINKI,DEPT ANAT,SF-00014 HELSINKI,FINLAND
[3] UNIV TURKU,DEPT MED BIOCHEM,SF-20520 TURKU,FINLAND
[4] UNIV TURKU,MEDCITY RES LAB,SF-20520 TURKU,FINLAND
[5] UNIV TURKU,DEPT VIROL,SF-20520 TURKU,FINLAND
关键词
D O I
10.1006/viro.1994.1494
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Attachment and entry of coxsackievirus A9 (CAV-9) to GMK cells were previously shown to be dependent on an arginine-glycine-aspartic acid (RGD) motif in the capsid protein VP1, suggesting integrins as candidate receptors for the virus. We have pursued the matter further and show that antibodies specific for the alpha(v) and/or beta(3) integrin subunits protect GMK cells from CAV-9 infection. Affinity purification of radioiodinated cell surface proteins using CAV-9 or virus-specific peptide (RRRGDL) columns confirmed that the alpha(v) beta(3), heterodimer, known as the vitronectin receptor, is recognized by the virus in GMK cells. Other proteins, of lower molecular weight (less than 40 kDa), were also bound to and specifically eluted from the columns, but their possible role in attachment and entry of CAV-9 remains to be elucidated by further studies. Of several other related viruses studied, only echovirus 22, which also has an RGD motif in the VP1 capsid protein, was found to compete for cell surface binding with CAV-9. (C) 1994 Academic Press, Inc.
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页码:357 / 365
页数:9
相关论文
共 35 条
  • [1] MANY RHINOVIRUS SEROTYPES SHARE THE SAME CELLULAR RECEPTOR
    ABRAHAM, G
    COLONNO, RJ
    [J]. JOURNAL OF VIROLOGY, 1984, 51 (02) : 340 - 345
  • [2] BAXT B, 1990, Virus Genes, V4, P73, DOI 10.1007/BF00308567
  • [3] INVOLVEMENT OF CELLULAR ADHESION SEQUENCES IN THE ATTACHMENT OF ADENOVIRUS TO THE HELA-CELL SURFACE
    BELIN, MT
    BOULANGER, P
    [J]. JOURNAL OF GENERAL VIROLOGY, 1993, 74 : 1485 - 1497
  • [4] INFECTION BY ECHOVIRUSES-1 AND ECHOVIRUSES-8 DEPENDS ON THE ALPHA-2 SUBUNIT OF HUMAN VLA-2
    BERGELSON, JM
    STJOHN, N
    KAWAGUCHI, S
    CHAN, M
    STUBDAL, H
    MODLIN, J
    FINBERG, RW
    [J]. JOURNAL OF VIROLOGY, 1993, 67 (11) : 6847 - 6852
  • [5] IDENTIFICATION OF THE INTEGRIN VLA-2 AS A RECEPTOR FOR ECHOVIRUS-1
    BERGELSON, JM
    SHEPLEY, MP
    CHAN, BMC
    HEMLER, ME
    FINBERG, RW
    [J]. SCIENCE, 1992, 255 (5052) : 1718 - 1720
  • [6] THE INTEGRIN VLA-2 BINDS ECHOVIRUS 1 AND EXTRACELLULAR-MATRIX LIGANDS BY DIFFERENT MECHANISMS
    BERGELSON, JM
    CHAN, BMC
    FINBERG, RW
    HEMLER, ME
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (01) : 232 - 239
  • [7] THE NUCLEOTIDE-SEQUENCE OF COXSACKIEVIRUS-A9 - IMPLICATIONS FOR RECEPTOR-BINDING AND ENTEROVIRUS CLASSIFICATION
    CHANG, KH
    AUVINEN, P
    HYYPIA, T
    STANWAY, G
    [J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 3269 - 3280
  • [8] THE NUCLEOTIDE-SEQUENCES OF WILD-TYPE COXSACKIEVIRUS-A9 STRAINS IMPLY THAT AN RGD MOTIF IN VP1 IS FUNCTIONALLY SIGNIFICANT
    CHANG, KH
    DAY, C
    WALKER, J
    HYYPIA, T
    STANWAY, G
    [J]. JOURNAL OF GENERAL VIROLOGY, 1992, 73 : 621 - 626
  • [9] CHERESH DA, 1987, J BIOL CHEM, V262, P1434
  • [10] INTEGRIN ALPHA-IIB-BETA-3 MEDIATES BINDING OF THE LYME-DISEASE AGENT BORRELIA-BURGDORFERI TO HUMAN PLATELETS
    COBURN, J
    LEONG, JM
    ERBAN, JK
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) : 7059 - 7063