EFFECT OF STIRRER SPEED AND BUFFERING AGENTS ON THE PRODUCTION OF GLUCOSE-OXIDASE AND CATALASE BY PENICILLIUM VARIABILE (P16) IN BENCHTOP BIOREACTOR

被引：49

作者：

PETRUCCIOLI, M

FENICE, M

PICCIONI, P

FEDERICI, F

机构：

[1] UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,I-01100 VITERBO,ITALY

[2] UNIV BASILICATA,DIPARTIMENTO BIOL DIFESA & BIOTECNOL AGROFORESTAL,I-85100 POTENZA,ITALY

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 04期

关键词：

GLUCOSE OXIDASE; CATALASE; PENICILLIUM VARIABILE; PRODUCTION; AGITATION; BUFFERING AGENTS;

D O I：

10.1016/0141-0229(94)00030-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Batch cultures of Penicillium variabile P16 were grown in a 3-1 benchtop bioreactor. Glucose oxidase and catalase production was followed while varying stirrer speed and adding buffering agents. Four hundred rev min(-1) appeared to be optimum to ensure good growth and best enzyme production; above this, production was reduced. Among the different chemicals (CaCO3, NaOH, and NaOH plus CaCl2) employed to counteract the pH drop in the fermentation broth, CaCO3 gave the best results. The time course of growth, enzyme activities, and gluconate production at 400 rev min(-1) and using CaCO3 as neutralizing agent is reported.

引用

页码：336 / 339

页数：4

共 21 条

[1]

Barker S.A., 1980, MICROBIAL ENZYMES BI, P173

[2] GLUCOSE-OXIDASE CATALASE IMPROVES PRESERVATION OF SHRIMP (HETEROCARPUS-REEDI) [J].