THE REGULATORY C-PROTEINS FROM DIFFERENT RESTRICTION-MODIFICATION SYSTEMS CAN CROSS-COMPLEMENT

被引：29

作者：

IVES, CL

SOHAIL, A

BROOKS, JE

机构：

[1] NEW ENGLAND BIOLABS INC,BEVERLY,MA 01915

[2] PUNJAB UNIV,CEMB,LABORE 53700,PAKISTAN

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 21期

关键词：

D O I：

10.1128/jb.177.21.6313-6315.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes Com other systems were tested in vivo for their ability to cross-complement. C.BamHI protein aas identified, purified, and used to raise polyclonal antibodies. Attempts to detect other C proteins in cell extracts by cross-reactivity with C.BamHI antibodies proved unsuccessful.

引用

收藏

页码：6313 / 6315

页数：3

相关论文

共 21 条

[1] CHARACTERIZATION OF THE GENES-CODING FOR THE ECO RV RESTRICTION AND MODIFICATION SYSTEM OF ESCHERICHIA-COLI [J].

BOUGUELERET, L ;

SCHWARZSTEIN, M ;

TSUGITA, A ;

ZABEAU, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (08) :3659-3676

[2] CLONING THE BAMHI RESTRICTION MODIFICATION SYSTEM [J].

BROOKS, JE ;

BENNER, JS ;

HEITER, DF ;

SILBER, KR ;

SZNYTER, LA ;

JAGERQUINTON, T ;

MORAN, LS ;

SLATKO, BE ;

WILSON, GG ;

NWANKWO, DO .

NUCLEIC ACIDS RESEARCH, 1989, 17 (03) :979-997

[3] CHARACTERIZATION OF THE CLONED BAMHI RESTRICTION MODIFICATION SYSTEM - ITS NUCLEOTIDE-SEQUENCE, PROPERTIES OF THE METHYLASE, AND EXPRESSION IN HETEROLOGOUS HOSTS [J].

BROOKS, JE ;

NATHAN, PD ;

LANDRY, D ;

SZNYTER, LA ;

WAITEREES, P ;

IVES, CL ;

MORAN, LS ;

SLATKO, BE ;

BENNER, JS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (04) :841-850

[4] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[5] CLONING, CHARACTERIZATION AND HETEROLOGOUS EXPRESSION OF THE SMAI RESTRICTION-MODIFICATION SYSTEM [J].

HEIDMANN, S ;

SEIFERT, W ;

KESSLER, C ;

DOMDEY, H .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9783-9796

[6] REGULATION OF THE BAMHI RESTRICTION-MODIFICATION SYSTEM BY A SMALL INTERGENIC OPEN READING FRAME, BAMHIC, IN BOTH ESCHERICHIA-COLI AND BACILLUS-SUBTILIS [J].

IVES, CL ;

NATHAN, PD ;

BROOKS, JE .

JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7194-7201

[7] OVEREXPRESSION, PURIFICATION AND CRYSTALLIZATION OF BAMHI ENDONUCLEASE [J].

JACK, WE ;

GREENOUGH, L ;

DORNER, LF ;

XU, SY ;

STRZELECKA, T ;

AGGARWAL, AK ;

SCHILDKRAUT, I .

NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1825-1829

[8] CHARACTERIZATION OF CLONES OF THE BAMHI METHYLTRANSFERASE GENE [J].

NATHAN, PD ;

BROOKS, JE .

GENE, 1988, 74 (01) :35-36

[9] RECOGNITION SEQUENCE OF SPECIFIC ENDONUCLEASE BAMHI FROM BACILLUS-AMYLOLIQUEFACIENS-H [J].

ROBERTS, RJ ;

WILSON, GA ;

YOUNG, FE .

NATURE, 1977, 265 (5589) :82-84

[10]

Sambrook J., 1989, MOL CLONING LAB MANU