MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA FOR BOVINE PHOSPHOLIPASE C-ALPHA - PROPOSAL OF REDESIGNATION OF PHOSPHOLIPASE C-ALPHA

被引：26

作者：

HIRANO, N

SHIBASAKI, F

KATO, H

SAKAI, R

TANAKA, T

NISHIDA, J

YAZAKI, Y

TAKENAWA, T

HIRAI, H

机构：

[1] UNIV TOKYO,DEPT INTERNAL MED 3,TOKYO 113,JAPAN

[2] JICHI MED SCH,DEPT BIOL MOLEC,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[3] TOKYO METROPOLITAN GERIATR HOSP & INST GERENTOL,DEPT BIOL RES,TOKYO 173,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated bovine phospholipase C (PLC)-alpha cDNA from bovine thymus. Sequence analysis showed that PLC-a is highly conserved among rat, mouse, and calf and that it has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved in the mammals. Southern blot analysis revealed that bovine PLC-a is derived from a single gene. When PLC-and cDNA was stably transfected in NIH3T3 cells, there was no increase in PLC activity. PLC-alpha is supposed to be a member not of PLC superfamily but of Trp-Cys-Gly-His-Cys-Lys motif-containing proteins consisting of protein disulfide isomerase, P5, ERp72, and thioredoxin. PLC-alpha should be redesignated ERp57 (ER-resided p57). (C) 1994 Academic Press, Inc.

引用

页码：375 / 382

页数：8

共 22 条

[1] MOLECULAR-CLONING AND COMPLETE AMINO-ACID-SEQUENCE OF FORM-I PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C [J].