EFFECTS OF A FISH-OIL LARD DIET ON RAT PLASMA-LIPOPROTEINS, LIVER FAS, AND LIPOLYTIC ENZYMES

被引:49
作者
BENHIZIA, F
HAINAULT, I
SEROUGNE, C
LAGRANGE, D
HAJDUCH, E
GUICHARD, C
MALEWIAK, MI
QUIGNARDBOULANGE, A
LAVAU, M
GRIGLIO, S
机构
[1] INSERM, U177, UNITE PATHOPHYSIOL NUTR, F-75006 PARIS, FRANCE
[2] UNIV PARIS 11, PHYSIOL NUTR LAB, F-91405 ORSAY, FRANCE
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1994年 / 267卷 / 06期
关键词
LIPOPROTEIN LIPASE; HEPATIC LIPASE; FATTY ACID SYNTHASE; 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE; LIPOGENESIS; CHOLESTEROL; GENE EXPRESSION; ADIPOSE TISSUE; VERY-LOW-DENSITY LIPOPROTEIN; LOW-DENSITY LIPOPROTEIN; HIGH-DENSITY LIPOPROTEIN;
D O I
10.1152/ajpendo.1994.267.6.E975
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The effects of a fish oil concentrate on blood lipids and lipoproteins were examined in relation to their effects on liver fatty acid synthase (FAS), 3-hydroxy-3methylglutaryl-CoA (HMG-CoA) reductase, adipose tissue lipoprotein lipase (LPL), and hepatic triglyceride lipase (H-TGL). For 15 days, a-mo-old rats were fed a control diet (10% of calories from fat, 4% fat by weight) or diets with 50% of calories (25% wt/wt) provided by lard, lard and fish oil concentrate (35%/15%), or lard and corn oil (35%/15%). The high-lard diet increased plasma chylomicron and liver triglycerides. The high-lard diet greatly decreased FAS, HMG-CoA reductase, and LPL activities; it also reduced H-TGL activity. Compared with the lard diet, the lard-fish oil diet decreased plasma TG by drastically lowering chylomicron (4-fold, P < 0.001) and very-low-density lipoprotein levels(P < 0.001). It also reduced high-density lipoprotein levels. The lard-fish oil diet prevented hepatic triglyceride accumulation and decreased FAS activity and mass by 3.5-fold (P < 0.001) but did not further decrease HMG-CoA reductase activity. Adipose tissue LPL activity was 2.5-fold (P < 0.001) higher with the lard-fish oil diet than with the lard diet, and H-TGL activity decreased significantly (-32%, P < 0.01), despite unaltered levels of H-TGL mRNA. These effects were significant with only 10% fish oil concentrate in the lard diet. They were not observed viith the lard-corn oil diet. Thus inclusion of fish oil concentrate in a lard diet results in a marked decrease in plasma chylomicron and very-low-density lipoprotein levels associated with an almost total suppression of hepatic FAS and a sustained adipose tissue LPL activity. The decreased H-TGL activity may be a secondary adaptation to the induced hypolipidemia or may result from a specific posttranscriptional inhibition of H-TGL gene expression by fish oil n-3 fatty acids.
引用
收藏
页码:E975 / E982
页数:8
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