ADENOVIRUS PROTEIN ASSOCIATED WITH THE ENDS OF REPLICATING DNA-MOLECULES

被引：44

作者：

STILLMAN, BW

BELLETT, AJD

机构：

[1] Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra

来源：

VIROLOGY | 1979年 / 93卷 / 01期

关键词：

D O I：

10.1016/0042-6822(79)90276-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The adenovirus DNA-protein complex contains the linear DNA molecule and a 55K protein covalently attached to each 5′ end. We describe a simple assay for adenovirus DNA-protein complex, in which DNA, covalently linked to the terminal protein, specifically binds to benzoylated naphthoylated DEAE-cellulose. The DNA-protein complex can be recovered intact from the column by elution with urea and SDS, or the DNA moiety can be eluted after incubation of the column with protease. Using this assay system, we have shown that protein is associated with pulse-labeled single strands of DNA from the terminal restriction enzyme fragments of replicating adenovirus DNA. We suggest that the terminal protein has a function in DNA replication. © 1979.

引用

页码：69 / 79

页数：11

共 42 条

[11] BIDIRECTIONAL REPLICATION OF ADENOVIRUS TYPE-2 DNA [J].

HORWITZ, MS .

JOURNAL OF VIROLOGY, 1976, 18 (01) :307-315

[12] INTERMEDIATES IN SYNTHESIS OF TYPE-2 ADENOVIRUS DEOXYRIBONUCLEIC ACID [J].

HORWITZ, MS .

JOURNAL OF VIROLOGY, 1971, 8 (05) :675-&

[13] STRUCTURAL-ANALYSIS OF VIRAL REPLICATIVE INTERMEDIATES ISOLATED FROM ADENOVIRUS TYPE-2-INFECTED HELA-CELL NUCLEI [J].

KEDINGER, C ;

BRISON, O ;

PERRIN, F ;

WILHELM, J .

JOURNAL OF VIROLOGY, 1978, 26 (02) :364-379

[14] VISUALIZATION OF A CIRCULAR DNA-PROTEIN COMPLEX FROM ADENOVIRIONS [J].

KEEGSTRA, W ;

VANWIELINK, PS ;

SUSSENBACH, JS .

VIROLOGY, 1977, 76 (01) :444-447

[15] ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .6. INFLUENCE OF BACTERIOPHAGE-T2 ON THE SYNTHETIC PATHWAY IN HOST CELLS [J].

KORNBERG, A ;

ZIMMERMAN, SB ;

KORNBERG, SR ;

JOSSE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1959, 45 (06) :772-785

[16] MULTIPLE STAGES IN ENZYMIC REPLICATION OF DNA [J].

KORNBERG, A .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1977, 5 (02) :359-&

[17] ISOLATION AND PARTIAL CHARACTERIZATION OF SINGLE-STRANDED ADENOVIRAL DNA PRODUCED DURING SYNTHESIS OF ADENOVIRUS TYPE-2 DNA [J].

LAVELLE, G ;

PATCH, C ;

KHOURY, G ;

ROSE, J .

JOURNAL OF VIROLOGY, 1975, 16 (04) :775-782

[18] STRUCTURE OF REPLICATING ADENOVIRUS-2 DNA-MOLECULES [J].

LECHNER, RL ;

KELLY, TJ .

CELL, 1977, 12 (04) :1007-1020

[19]

Levine A J, 1976, Curr Top Microbiol Immunol, V73, P67

[20] CLEAVAGE MAPS OF DNA FROM ADENOVIRUS TYPES 2 AND 5 BY RESTRICTION ENDONUCLEASES ECORI AND HPAI [J].

MULDER, C ;

ARRAND, JR ;

DELIUS, H ;

KELLER, W ;

PETTERSSON, U ;

ROBERTS, RJ ;

SHARP, PA .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1974, 39 :397-400

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