STIMULATION OF INOSITOL PHOSPHATE PRODUCTION AND GTPASE ACTIVITY BY COMPOUND-48/80 IN RAT PERITONEAL MAST-CELLS

被引：23

作者：

WU, CY ^{[1
]}

CHEN, CF ^{[1
]}

CHIANG, CF ^{[1
]}

机构：

[1] NATL YANG MING MED COLL,DEPT PHARMACOL,TAIPEI 112,TAIWAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 192卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1401

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase C activity, GTPase activity and cytosolic-free calcium concentration in mast cells were stimulated by compound 48/80. Accumulation of inositol phosphates in rat mast cells was stimulated by guanosine 5′-[γ-thio]-triphosphate. Guanosine 5′-[γ-thio]- triphosphate, however, exhibited no effect upon the purified phospholipase C activity and upon phospholipase C in the mast cell homogenate. The stimulatory effect of compound 48/80 upon phospholipase C activity of intact mast cells was observed to have been well correlated with that on GTPase activity of mast cell homogenate. Compound 48/80 exhibited no effect upon the binding of radioactive guanosine 5′-[γ-thio]- triphosphate to mast cell homogenate. Phospholipase C activity was verified by the above results to become affected by compound 48/80 through guanine nucleotide-binding regulatory protein. © 1993 Academic Press.

引用

页码：204 / 213

页数：10

共 32 条

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