ANALYSIS OF HEPATITIS-C VIRUS GENOTYPES BY A LINE PROBE ASSAY AND CORRELATION WITH ANTIBODY PROFILES

被引：51

作者：

VANDOORN, LJ

KLETER, B

STUYVER, L

MAERTENS, G

BROUWER, H

SCHALM, S

HEIJTINK, R

QUINT, W

机构：

[1] ERASMUS UNIV ROTTERDAM,DEPT VIROL,3000 DR ROTTERDAM,NETHERLANDS

[2] NV INNOGENET SA,GHENT,BELGIUM

[3] ACAD HOSP DIJZIGT,ROTTERDAM,NETHERLANDS

来源：

JOURNAL OF HEPATOLOGY | 1994年 / 21卷 / 01期

关键词：

DIRECT SEQUENCING; LINE IMMUNE ASSAY; POLYMERASE CHAIN REACTION; REVERSE HYBRIDIZATION; RIBA;

D O I：

10.1016/S0168-8278(94)80148-7

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The 5' untranslated regions derived from 54 patients with a chronic hepatitis C virus infection were analyzed to determine the (sub)type of hepatitis C virus. Labelled polymerase chain reaction products from 5' untranslated region were used as probes for reverse hybridization in a line probe assay (Inno-LiPA) and results were validated by comparison with direct sequencing data. Five different genoypes could be distinguished based on 5' untranslated region sequence diversity. Results of typing by line probe assay and direct sequencing were similar. Antibody responses against core, NS-3, NS-4 and NS-5 epitopes were detected by RIBA-4 and Inno-LIA HCVAb II confirmatory assays. There was no consistent correlation between genotype and anti-HCV responses, although types 2, 3 and 4 hepatitis C virus isolates show poor reactivity with NS-4 epitopes. (C) Journal of Hepatology.

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页码：122 / 129

页数：8

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