THE SOYBEAN GH2/4 GENE THAT ENCODES A GLUTATHIONE-S-TRANSFERASE HAS A PROMOTER THAT IS ACTIVATED BY A WIDE-RANGE OF CHEMICAL-AGENTS

被引:86
作者
ULMASOV, T [1 ]
OHMIYA, A [1 ]
HAGEN, G [1 ]
GUILFOYLE, T [1 ]
机构
[1] UNIV MISSOURI, DEPT BIOCHEM, COLUMBIA, MO 65211 USA
关键词
D O I
10.1104/pp.108.3.919
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transcriptional activation of the soybean (Glycine max) GH2/4 gene (also referred to as Gmhsp 26-A) and increase in abundance of the GH2/4 mRNA (also referred to as pCE54) have been previously shown to occur following treatment of soybean seedlings with auxins, nonauxin analogs, heavy metals, and a variety of other agents. To determine whether the GH2/4 promoter is responsive to an array of different agents, we have analyzed the inducibility of the GH2/4 promoter fused to the P-glucuronidase reporter gene in transgenic tobacco (Nicotiana tabacum) plants. We have shown that a wide variety of chemical agents induce this promoter in a tissue-specific and concentration-dependent manner. In addition, we have used an affinity-purified antibody raised against recombinant GH2/4 protein to show that the GH2/4 protein increases in response to auxin application and is localized in the cytosol of soybean cells. Recombinant GH2/4 protein can be purified to homogeneity on a glutathione-agarose resin, and the purified protein has glutathione S-transferase activity when assayed with the substrate 1-chloro-2,4-dinitrobenzene.
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收藏
页码:919 / 927
页数:9
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