EXTRACELLULAR ATP ITSELF ELICITS SUPEROXIDE GENERATION IN GUINEA-PIG PERITONEAL-MACROPHAGES

被引：17

作者：

NAKANISHI, M ^{[1
]}

TAKIHARA, H ^{[1
]}

MINORU, Y ^{[1
]}

YAGAWA, K ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DIS CHEST RES INST,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

FEBS LETTERS | 1991年 / 282卷 / 01期

关键词：

MACROPHAGE; ATP RECEPTOR; SUPEROXIDE GENERATION; CA2+]I MOBILIZATION;

D O I：

10.1016/0014-5793(91)80451-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization. Pre-exposure to a small amount of phorbol myristate acetate (PMA) primed the ATP-induced generation of O2- without a change of [Ca2+]i. The results suggest that ATP-induced O2- generation is mediated by [Ca2+]i mobilization and by PT-sensitive G protein.

引用

页码：91 / 94

页数：4

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