MEASUREMENT OF TRANSLATIONAL ACCURACY INVIVO - MISSENSE REPORTING USING INACTIVE ENZYME MUTANTS

被引：6

作者：

CORNUT, B ^{[1
]}

WILLSON, RC ^{[1
]}

机构：

[1] UNIV HOUSTON, DEPT CHEM ENGN, HOUSTON, TX 77204 USA

来源：

BIOCHIMIE | 1991年 / 73卷 / 12期

关键词：

MISTRANSLATION; MISSENSE; RIBOSOME; TRYPTOPHAN SYNTHETASE; LUCIFERASE;

D O I：

10.1016/0300-9084(91)90192-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.

引用

页码：1567 / 1572

页数：6