ASSOCIATION OF HIGH-MOLECULAR-WEIGHT DNA FRAGMENTATION WITH APOPTOTIC OR NON-APOPTOTIC CELL-DEATH INDUCED BY CALCIUM IONOPHORE

被引：34

作者：

KATAOKA, A

KUBOTA, M

WAKAZONO, Y

OKUDA, A

BESSHO, R

LIN, YW

USAMI, I

AKIYAMA, Y

FURUSHO, K

机构：

[1] Department of Pediatrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-01, 54 Kawahara-cho, Shogoin

来源：

FEBS LETTERS | 1995年 / 364卷 / 03期

关键词：

APOPTOSIS; NECROSIS; HIGH MOLECULAR WEIGHT DNA FRAGMENTATION; INTERNUCLEOSOMAL DNA FRAGMENTATION; ENDONUCLEASE;

D O I：

10.1016/0014-5793(95)00405-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines, An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DIVA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions, Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines, The activity was more dependent on Mg2+ than Ca2+ in HL-60, whereas it was Ca2+-dependent in MOLT-and These results suggest that HMW DNA fragmentation is not specific to apoptosis.

引用

页码：264 / 267

页数：4

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