ASSOCIATION OF HIGH-MOLECULAR-WEIGHT DNA FRAGMENTATION WITH APOPTOTIC OR NON-APOPTOTIC CELL-DEATH INDUCED BY CALCIUM IONOPHORE

被引：34

作者：

KATAOKA, A

KUBOTA, M

WAKAZONO, Y

OKUDA, A

BESSHO, R

LIN, YW

USAMI, I

AKIYAMA, Y

FURUSHO, K

机构：

[1] Department of Pediatrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-01, 54 Kawahara-cho, Shogoin

来源：

FEBS LETTERS | 1995年 / 364卷 / 03期

关键词：

APOPTOSIS; NECROSIS; HIGH MOLECULAR WEIGHT DNA FRAGMENTATION; INTERNUCLEOSOMAL DNA FRAGMENTATION; ENDONUCLEASE;

D O I：

10.1016/0014-5793(95)00405-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines, An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DIVA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions, Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines, The activity was more dependent on Mg2+ than Ca2+ in HL-60, whereas it was Ca2+-dependent in MOLT-and These results suggest that HMW DNA fragmentation is not specific to apoptosis.

引用

页码：264 / 267

页数：4

共 20 条

[11]

OBERHAMMER F, 1993, J CELL SCI, V104, P317

[12] APOPTOTIC DEATH IN EPITHELIAL-CELLS - CLEAVAGE OF DNA TO 300 AND OR 50 KB FRAGMENTS PRIOR TO OR IN THE ABSENCE OF INTERNUCLEOSOMAL FRAGMENTATION [J].

OBERHAMMER, F ;

WILSON, JW ;

DIVE, C ;

MORRIS, ID ;

HICKMAN, JA ;

WAKELING, AE ;

WALKER, PR ;

SIKORSKA, M .

EMBO JOURNAL, 1993, 12 (09) :3679-3684

[13] CISPLATIN INDUCES APOPTOSIS IN A HUMAN OVARIAN-CARCINOMA CELL-LINE WITHOUT CONCOMITANT INTERNUCLEOSOMAL DEGRADATION OF DNA [J].

ORMEROD, MG ;

ONEILL, CF ;

ROBERTSON, D ;

HARRAP, KR .

EXPERIMENTAL CELL RESEARCH, 1994, 211 (02) :231-237

[14] CA-2+-DEPENDENT MECHANISMS OF CYTOTOXICITY AND PROGRAMMED CELL-DEATH [J].

ORRENIUS, S ;

MCCABE, MJ ;

NICOTERA, P .

TOXICOLOGY LETTERS, 1992, 64-5 :357-364

[15]

PEITSCH MC, 1994, TRENDS CELL BIOL, V2, P262

[16] INHIBITION OF BOTH ETOPOSIDE-INDUCED DNA FRAGMENTATION AND ACTIVATION OF POLY(ADP-RIBOSE) SYNTHESIS BY ZINC ION [J].

SHIMIZU, T ;

KUBOTA, M ;

TANIZAWA, A ;

SANO, H ;

KASAI, Y ;

HASHIMOTO, H ;

AKIYAMA, Y ;

MIKAWA, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 169 (03) :1172-1177

[17] SEPARATE METABOLIC PATHWAYS LEADING TO DNA FRAGMENTATION AND APOPTOTIC CHROMATIN CONDENSATION [J].

SUN, DY ;

JIANG, S ;

ZHENG, LM ;

OJCIUS, DM ;

YOUNG, JDE .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (02) :559-568

[18] MULTIPLE FORMS OF NUCLEAR DEOXYRIBONUCLEASE IN RAT THYMOCYTES [J].

TANUMA, S ;

SHIOKAWA, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 203 (02) :789-797

[19] THAPSIGARGIN, A TUMOR PROMOTER, DISCHARGES INTRACELLULAR CA-2+ STORES BY SPECIFIC-INHIBITION OF THE ENDOPLASMIC-RETICULUM CA-2+-ATPASE [J].

THASTRUP, O ;

CULLEN, PJ ;

DROBAK, BK ;

HANLEY, MR ;

DAWSON, AP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2466-2470

[20]

WALKER PR, 1991, CANCER RES, V51, P1078

← 1 2 →