FOLDING, FLAVINYLATION, AND MITOCHONDRIAL IMPORT OF 6-HYDROXY-D-NICOTINE OXIDASE FUSED TO THE PRESEQUENCE OF RATE DIMETHYLGLYCINE DEHYDROGENASE

We analyzed the folding, covalent flavinylation, and mitochondrial import of the rabbit reticulocyte lysate-translated bacterial 6-hydroxy-D-nicotine oxidase (6-HDNO) fused to the mitochondrial targeting sequence of rat Liver dimethylglycine dehydrogenase, Translation of 6-HDNO in FAD-supplemented reticulocyte lysate resulted in a protein that contained covalently incorporated FED, exhibited enzyme activity, and was trypsin-resistant, a characteristic of the tight conformation of the holoenzyme, The attached mitochondrial presequence did not prevent folding, binding of FAD, or enzyme activity of the 6-HDNO moiety of the fusion protein (pre-6-HDNO), Pre-6-HDNO was imported into rat liver mitochondria and processed by the mitochondrial processing peptidase, Incubation of the trypsin-resistant pre-holo-6-HDNO protein with deenergized rat Liver mitochondria demonstrated that upon contact with mitochondria, the protein was unfolded and became trypsin sensitive. Mitochondrial import assays showed that the unfolded pre-holo-6-HDNO with covalently attached FAD was imported into rat Liver mitochondria, Inside the mitochondrion the holo-6-HDNO was refolded into the trypsin-resistant conformation, However, when pre-apo-6-HDNO was imported only part of the protein became trypsin resistant (approximately 20%). Addition of FAD and the allosteric effector glycerol 3-phosphate to apo-6-HDNO containing mitochondrial matrix was required to transform the protein into the trypsin-resistant conformation characteristic of holo-6-HDNO,