SEPARATION OF OLIGOMANNOSE-TYPE SUGAR CHAINS HAVING ONE TO 5 MANNOSE RESIDUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AS THEIR PYRIDYLAMINO DERIVATIVES

被引：44

作者：

OKU, H

HASE, S

IKENAKA, T

机构：

[1] Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90303-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten oligomannose-type sugar chains (ManGlcNAc2 ∼ Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the component PA-sugar chains by reversed-phase HPLC on a Capcell Pak C18 column according to their chemical structures. The method is useful for studying the substrate specificities of α-mannosidases with Man5GlcNAc2-PA as a substrate. © 1990.

引用

页码：331 / 334

页数：4

共 12 条

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