PERMEATION OF PROTEINS FROM THE BLOOD INTO PERIPHERAL-NERVES AND GANGLIA

被引:55
作者
ALLEN, DT [1 ]
KIERNAN, JA [1 ]
机构
[1] UNIV WESTERN ONTARIO,DEPT ANAT,LONDON N6A 5C1,ON,CANADA
关键词
D O I
10.1016/0306-4522(94)90192-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have attempted to resolve apparently conflicting observations of previous investigators regarding the penetration of proteins into peripheral ganglia and nerves of the rat. Horseradish peroxidase, which is largely cleared from the blood and extracellular fluids in less than 30 min, entered all the extracellular spaces of ganglia, including the clefts between glial cells and neurons, but it did not enter the endoneurium. Rhodamine B-conjugated bovine albumin quickly entered sensory and sympathetic ganglia, but its penetration into avascular enteric nervous tissue was arrested at the outer margin of each myenteric and submucosal ganglion. If injected daily for a week, this fluorescent protein was seen also in the endoneurium, but it was still absent from enteric ganglia. The failure to enter enteric ganglia may have been due to the entrapment of aggregates of dye-labelled albumin molecules in the basal lamina that encloses the enteric nervous system. Extracellular endogenous albumin immunoreactivity was seen in all parts of peripheral nerves and in all types of ganglion. Some neuronal perikarya contained albumin-immunoreactive material, the strongest staining was in enteric neurons. Albumin may reach these cell bodies by retrograde axonal transport from peripheral terminals. We conclude that all the extracellular spaces of the rat's peripheral nervous system are accessible to plasma proteins, though diffusion occurs more slowly into the endoneurium than into ganglia.
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页码:755 / 764
页数:10
相关论文
共 47 条
[1]  
Allen D T, 1990, Neuroreport, V1, P33, DOI 10.1097/00001756-199009000-00010
[2]  
ALLEN DT, 1992, THESIS U W ONTARIO L
[3]   CELLULAR UPTAKE OF EXOGENOUS HORSERADISH-PEROXIDASE IN MOUSE PERIPHERAL-NERVE [J].
ARVIDSON, B .
ACTA NEUROPATHOLOGICA, 1977, 37 (01) :35-41
[4]   STUDY OF THE PERINEURIAL DIFFUSION BARRIER OF A PERIPHERAL GANGLION [J].
ARVIDSON, B .
ACTA NEUROPATHOLOGICA, 1979, 46 (1-2) :139-144
[5]   VASCULAR-PERMEABILITY TO FLUORESCENT PROTEIN TRACER IN TRIGEMINAL NERVE AND GASSERIAN GANGLION [J].
ARVIDSSON, B ;
KRISTENSSON, K ;
OLSSON, Y .
ACTA NEUROPATHOLOGICA, 1973, 26 (03) :199-205
[6]   PERMEABILITY OF THE NORMAL RAT-BRAIN, SPINAL-CORD AND DORSAL-ROOT GANGLIA MICROCIRCULATIONS TO IMMUNOGLOBULINS-G [J].
AZZI, G ;
BERNAUDIN, JF ;
BOUCHAUD, C ;
BELLON, B ;
FLEURYFEITH, J .
BIOLOGY OF THE CELL, 1990, 68 (01) :31-36
[7]   TRANSCYTOSIS OF PROTEIN THROUGH THE MAMMALIAN CEREBRAL EPITHELIUM AND ENDOTHELIUM .1. CHOROID-PLEXUS AND THE BLOOD CEREBROSPINAL-FLUID BARRIER [J].
BALIN, BJ ;
BROADWELL, RD .
JOURNAL OF NEUROCYTOLOGY, 1988, 17 (06) :809-826
[8]  
BISHOP MA, 1992, J ANAT, V181, P29
[9]   JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN VERTEBRATE BRAIN [J].
BRIGHTMA.MW ;
REESE, TS .
JOURNAL OF CELL BIOLOGY, 1969, 40 (03) :648-+
[10]   ULTRASTRUCTURE OF AUERBACHS PLEXUS IN GUINEA-PIG .2. NON-NEURONAL ELEMENTS [J].
COOK, RD ;
BURNSTOCK, G .
JOURNAL OF NEUROCYTOLOGY, 1976, 5 (02) :195-206