FUNCTIONAL DISSECTION OF THE DYNEIN MOTOR DOMAIN

被引：5

作者：

ESHEL, D

机构：

[1] Department of Biology, Brooklyn College, City University of New York, Brooklyn

来源：

CELL MOTILITY AND THE CYTOSKELETON | 1995年 / 32卷 / 02期

关键词：

CYTOPLASMIC DYNEIN; MOTOR DOMAIN; MUTATIONAL ANALYSIS;

D O I：

10.1002/cm.970320213

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The highly conserved lysine residue in the putative hydrolytic ATP-binding motif of the yeast cytoplasmic dynein heavy chain was replaced with leucine. The mutation was generated by a two-stage transformation method designed for genomic site-directed mutagenesis. Preliminary observations show that the effects of this alteration on the cellular roles of dynein are indistinguishable from those of a disruption mutation in which the entire motor domain is not expressed. (C) 1995 Wiley-Liss, Inc.

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页码：133 / 135

页数：3

相关论文

共 19 条

[1]

ASAI DJ, 1994, J CELL SCI, V107, P839

[2] A SIMPLE AND EFFICIENT METHOD FOR DIRECT GENE DELETION IN SACCHAROMYCES-CEREVISIAE [J].

BAUDIN, A ;

OZIERKALOGEROPOULOS, O ;

DENOUEL, A ;

LACROUTE, F ;

CULLIN, C .

NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3329-3330

[3]

CREMO CR, 1989, J BIOL CHEM, V264, P6608

[4] CYTOPLASMIC DYNEIN IS REQUIRED FOR NORMAL NUCLEAR SEGREGATION IN YEAST [J].

ESHEL, D ;

URRESTARAZU, LA ;

VISSERS, S ;

JAUNIAUX, JC ;

VANVLIETREEDIJK, JC ;

PLANTA, RJ ;

GIBBONS, IR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :11172-11176

[5] PHYLOGENY AND EXPRESSION OF AXONEMAL AND CYTOPLASMIC DYNEIN GENES IN SEA-URCHINS [J].

GIBBONS, BH ;

ASAI, DJ ;

TANG, WJY ;

HAYS, TS ;

GIBBONS, IR .

MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (01) :57-70

[6] MULTIPLE NUCLEOTIDE-BINDING SITES IN THE SEQUENCE OF DYNEIN BETA-HEAVY CHAIN [J].

GIBBONS, IR ;

GIBBONS, BH ;

MOCZ, G ;

ASAI, DJ .

NATURE, 1991, 352 (6336) :640-643

[7] BETA-SUBUNIT OF ESCHERICHIA-COLI F1-ATPASE - AN AMINO-ACID REPLACEMENT WITHIN A CONSERVED SEQUENCE (G-X-X-X-X-G-K-T/S) OF NUCLEOTIDE-BINDING PROTEINS [J].

HSU, SY ;

NOUMI, T ;

TAKEYAMA, M ;

MAEDA, M ;

ISHIBASHI, S ;

FUTAI, M .

FEBS LETTERS, 1987, 218 (02) :222-226

[8] DYNEIN FROM DICTYOSTELIUM - PRIMARY STRUCTURE COMPARISONS BETWEEN A CYTOPLASMIC MOTOR ENZYME AND FLAGELLAR DYNEIN [J].

KOONCE, MP ;

GRISSOM, PM ;

MCINTOSH, JR .

JOURNAL OF CELL BIOLOGY, 1992, 119 (06) :1597-1604

[9] DROSOPHILA CYTOPLASMIC DYNEIN, A MICROTUBULE MOTOR THAT IS ASYMMETRICALLY LOCALIZED IN THE OOCYTE [J].

LI, MG ;

MCGRAIL, M ;

SERR, M ;

HAYS, TS .

JOURNAL OF CELL BIOLOGY, 1994, 126 (06) :1475-1494

[10] DISRUPTION OF MITOTIC SPINDLE ORIENTATION IN A YEAST DYNEIN MUTANT [J].

LI, YY ;

YEH, E ;

HAYS, T ;

BLOOM, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10096-10100