TIME-LAPSE VIDEO RECORDINGS OF HIGHLY PURIFIED HUMAN HEMATOPOIETIC PROGENITOR CELLS IN CULTURE

被引:13
作者
DENKERS, IAM
DRAGOWSKA, W
JAGGI, B
PALCIC, B
LANSDORP, PM
机构
[1] BRITISH COLUMBIA CANC RES CTR,TERRY FOX LAB,601 W 10TH AVE,VANCOUVER V5Z 1L3,BC,CANADA
[2] UNIV BRITISH COLUMBIA,DEPT PATHOL,VANCOUVER V6T 1W5,BC,CANADA
[3] UNIV BRITISH COLUMBIA,BRITISH COLUMBIA CANC AGCY,VANCOUVER V6T 1W5,BC,CANADA
[4] UNIV BRITISH COLUMBIA,DEPT PATHOL,VANCOUVER V6T 1W5,BC,CANADA
[5] FREE UNIV AMSTERDAM,DEPT HEMATOL,1007 MC AMSTERDAM,NETHERLANDS
关键词
TIME LAPSE VIDEO RECORDINGS; HEMATOPOIETIC CELLS; CELL ANALYZER; CELL CULTURE;
D O I
10.1002/stem.5530110312
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Major hurdles in studies of stem cell biology include the low frequency and heterogeneity of human hematopoietic precursor cells in bone marrow and the difficulty of directly studying the effect of various culture conditions and growth factors on such cells. We have adapted the cell analyzer imaging system for monitoring and recording the morphology of limited numbers of cells under various culture conditions. Hematopoietic progenitor cells with a CD34+ CD45RA(lo) CD71lo phenotype were purified from previously frozen organ donor bone marrow by fluorescence activated cell sorting. Cultures of such cells were analyzed with the imaging system composed of an inverted microscope contained in an incubator, a video camera, an optical memory disk recorder and a computer-controlled motorized microscope XYZ precision stage. Fully computer-controlled video images at defined XYZ positions were captured at selected time intervals and recorded at a predetermined sequence on an optical memory disk. In this study, the cell analyzer system was used to obtain descriptions and measurements of hematopoietic cell behavior, like cell motility, cell interactions, cell shape, cell division, cell cycle time and cell size changes under different culture conditions.
引用
收藏
页码:243 / 248
页数:6
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