SYNTHETIC OLIGONUCLEOTIDE COCKTAILS AS PROBES FOR DETECTION OF HUMAN PARVOVIRUS B19

被引：9

作者：

CUBIE, HA

GRZYBOWSKI, J

DASILVA, C

DUNCAN, L

BROWN, T

SMITH, NM

机构：

[1] UNIV EDINBURGH,DEPT CHEM,EDINBURGH EH9 3JJ,MIDLOTHIAN,SCOTLAND

[2] ROYAL HOSP SICK CHILDREN,DEPT PAEDIAT PATHOL,EDINBURGH EH9 1LF,MIDLOTHIAN,SCOTLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 01期

关键词：

OLIGONUCLEOTIDES; ISH; PARVOVIRUS B19; DIG LABELING; DNP LABELING;

D O I：

10.1016/0166-0934(94)00179-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A cocktail of 10 oligonucleotides selected at intervals along the length of the genome of human parvovirus B19 was labelled enzymically with digoxigenin and chemically with either digoxigenin (DIG) or dinitrophenyl (DNP). Chemical labelling was easier and more practical for the production of large quantities of probe. Pools labelled with either digoxigenin or DNP could detect 10 fg of B19 DNA in a dot blot reaction using an alkaline phosphatase antibody conjugate and colorimetric detection. Formalin fixed tissue from 11 consecutive cases of fetal hydrops were examined by in situ hybridisation (ISH). Both probe cocktails detected human parvovirus B19 DNA in 3 cases, with positive cells in all tissues examined and with equal sensitivity. The DNP pool is significantly cheaper and simpler to produce and could provide an inexpensive reagent suitable for diagnostic detection of viral nucleic acid in histopathological material.

引用

页码：91 / 102

页数：12

共 17 条

[1] FAST SOLID SUPPORT DETECTION OF HUMAN PAPILLOMAVIRUS IN IN-VITRO AMPLIFIED DNA USING A DNP ANTI DNP MONOCLONAL-ANTIBODY COUPLE
BACCARDLONGERE, M
ALPHABAZIN, B
CHYPRE, C
SAUVAIGO, S
TEOULE, R
BERNARD, P
SEIGNEURIN, JM
[J]. JOURNAL OF VIROLOGICAL METHODS, 1994, 46 (01) : 29 - 38
[2] BROWN T, 1984, LANCET, V2, P1033
[3] SYNTHETIC OLIGONUCLEOTIDE PROBES FOR THE DETECTION OF HUMAN PAPILLOMA VIRUSES BY INSITU HYBRIDIZATION
CUBIE, HA
NORVAL, M
[J]. JOURNAL OF VIROLOGICAL METHODS, 1988, 20 (03) : 239 - 249
[4] APPLICATION OF MOLECULAR TECHNIQUES IN THE RAPID DIAGNOSIS OF EBV-ASSOCIATED ORAL HAIRY LEUKOPLAKIA
CUBIE, HA
FELIX, DH
SOUTHAM, JC
WRAY, D
[J]. JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1991, 20 (06) : 271 - 274
[5] DETECTION OF RESPIRATORY SYNCYTIAL VIRUS-ANTIGEN AND NUCLEIC-ACID IN CLINICAL SPECIMENS USING SYNTHETIC OLIGONUCLEOTIDES
CUBIE, HA
INGLIS, JM
MCGOWAN, AM
[J]. JOURNAL OF VIROLOGICAL METHODS, 1991, 34 (01) : 27 - 35
[6] CUBIE HA, 1994, IN PRESS MOL CELL PR
[7] SYNTHESIS AND ANTIBODY-MEDIATED DETECTION OF OLIGONUCLEOTIDES CONTAINING MULTIPLE 2,4-DINITROPHENYL REPORTER GROUPS
GRZYBOWSKI, J
WILL, DW
RANDALL, RE
SMITH, CA
BROWN, T
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (08) : 1705 - 1712
[8] HALLCJ, 1994, ARCH DIS CHILD, V71, pF4
[9] HEWLINS MJE, 1994, J IMMUNOL METHODS, V70, P111
[10] JASANI B, 1983, IMMUNOL COMMUN, V12, pA51

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