ENANTIOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF (SR)-MEFLOQUINE AND (RS)-MEFLOQUINE IN PLASMA USING N-BENZYLOXYCARBONYL-GLYCYL-L-PROLINE AS CHIRAL COUNTER ION

被引:14
作者
BERGQVIST, Y
ALKABBANI, J
PETTERSSON, C
HUYNH, NH
机构
[1] HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT CLIN PHARMACOL,TROP PHARMACOL UNIT,S-14186 HUDDINGE,SWEDEN
[2] UNIV UPPSALA,CTR BIOMED,DEPT ANALYT PHARMACEUT CHEM,S-75123 UPPSALA,SWEDEN
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 620卷 / 02期
关键词
D O I
10.1016/0378-4347(93)80007-Q
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A stereoselective HPLC method is described for the determination of (SR)-and (RS)-mefloquine in plasma. The direct chiral separation is carried out on a Hypercarb-S column (porous graphitised carbon) with N-benzyloxycarbonyl-glycyl-L-proline (L-ZGP) as a chiral counter-ion in a reversed-phase system. The sample work-up included protein precipitation by addition of zinc sulphate and acetonitrile followed by liquid-liquid extraction with methyl-tert.-butyl ether. After evaporation of the organic phase, the residue is dissolved in the mobile phase and injected onto the column. Analyses of the enantiomers in plasma after a single oral dose of mefloquine indicates that the pharmacokinetics of the two enantiomers are different. The method is validated by determining the absolute recovery, linearity, accuracy, precision and inter- and intra-assay variation.
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页码:217 / 224
页数:8
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