GENETIC STUDIES OF RIBOSOMAL PROTEINS IN ESCHERICHIA COLI .2. ALTERED 30S RIBOSOMAL PROTEIN COMPONENT SPECIFIC TO SPECTINOMYCIN RESISTANT MUTANTS

被引：30

作者：

DEKIO, S

TAKATA, R

机构：

[1] Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Kasumi-cho

来源：

MOLECULAR AND GENERAL GENETICS | 1969年 / 105卷 / 03期

关键词：

D O I：

10.1007/BF00337473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spectinomycin resistant (spcr) mutants were obtained by treating the cells of E. coli K12, W3637 with nitrosoguanidine. The compositions of ribosomal proteins were analyzed for six out of eleven such spcr-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 30s ribosomal subunit from all of the spcr-mutants was found to contain the altered 30-4 protein component, while no difference was detected in 50s ribosomal proteins between spcr and spcs bacteria. © 1969 Springer-Verlag.

引用

页码：219 / &

共 10 条

[1] CORESISTANCE TO NEOMYCIN AND KANAMYCIN BY MUTATIONS IN AN ESCHERICHIA COLI LOCUS THAT AFFECTS RIBOSOMES [J].

APIRION, D ;

SCHLESSINGER, D .

JOURNAL OF BACTERIOLOGY, 1968, 96 (03) :768-+

[2] MAPPING AND COMPLEMENTATION OF 3 GENES SPECIFYING 30S RIBOSOMAL COMPONENTS IN ESCHERICHIA COLI [J].

APIRION, D ;

SCHLESSINGER, D .

JOURNAL OF BACTERIOLOGY, 1968, 96 (04) :1431-+

[3] INHIBITION OF PROTEIN SYNTHESIS BY SPECTINOMYCIN [J].

DAVIES, J ;

ANDERSON, P ;

DAVIS, BD .

SCIENCE, 1965, 149 (3688) :1096-+

[4]

FLAKS JG, 1966, COLD SPRING HARBOR S, V28, P623

[5] CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI [J].

LEBOY, PS ;

COX, EC ;

FLAKS, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 52 (06) :1367-+

[6] ESCHERICHIA COLI - K12 RIBOSOMAL PROTEIN AND STREPTOMYCIN REGION OF CHROMOSOME [J].

MAYUGA, C ;

MEIER, D ;

WANG, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 33 (02) :203-&

[7] RIBOSOMAL PROTEINS OF BACTERIAL CELLS - STRAIN- AND SPECIES-SPECIFICITY [J].

OTAKA, E ;

ITOH, T ;

OSAWA, S .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (01) :93-&

[8] GENETIC STUDIES OF RIBOSOMAL PROTEINS IN ESCHERICHIA COLI .I. MUTANTS AND STRAINS HAVING 30S RIBOSOMAL SUBUNIT WITH ALTERED PROTEIN COMPONENTS [J].

TAKATA, R ;

DEKIO, S ;

OTAKA, E ;

OSAWA, S .

MOLECULAR AND GENERAL GENETICS, 1969, 105 (02) :113-&

[9] ROLE OR RIBOSOMAL PROTEIN FOR BINDING OF DIHYDROSTREPTOMYCIN TO RIBOSOMES [J].

TANAKA, Y ;

KAJI, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 32 (02) :313-+

[10] STREPTOMYCIN RESISTANCE MUTATION IN ESCHERICHIA COLI - ALTERED RIBOSOMAL PROTEIN [J].

TRAUB, P ;

NOMURA, M .

SCIENCE, 1968, 160 (3824) :198-+

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