A SIMPLE TOUCH-DOWN POLYMERASE CHAIN-REACTION FOR THE DETECTION OF CANINE PARVOVIRUS AND FELINE PANLEUKOPENIA VIRUS IN FECES

被引：116

作者：

SCHUNCK, B ^{[1
]}

KRAFT, W ^{[1
]}

TRUYEN, U ^{[1
]}

机构：

[1] UNIV MUNICH, MED ANIM CLIN 1, D-80539 MUNICH, GERMANY

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 55卷 / 03期

关键词：

CANINE PARVOVIRUS; FELINE PANLEUKOPENIA VIRUS; POLYMERASE CHAIN REACTION (PCR); DIAGNOSIS;

D O I：

10.1016/0166-0934(95)00069-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction (PCR) assay is described for the detection of parvovirus in feces of dogs and cats. A touch-down protocol was used which enabled the specific amplification of virion DNA from feces after a fast and simple boiling pretreatment. The sensitivity of PCR was as high as ten infectious particles per reaction which corresponds to a titer of about 10(3) infectious particles per gram of unprocessed feces. This renders the PCR about 10- to 100-foId more sensitive than electron microscopy, the standard method for parvovirus diagnosis. The very rapid and simple sample preparation recommends this PCR assay as an alternative technique for routine parvovirus diagnosis.

引用

收藏

页码：427 / 433

页数：7

相关论文

共 23 条

[1] APPLICATION OF THE POLYMERASE CHAIN-REACTION (PCR) IN VETERINARY DIAGNOSTIC VIROLOGY [J].

BELAK, S ;

BALLAGIPORDANY, A .

VETERINARY RESEARCH COMMUNICATIONS, 1993, 17 (01) :55-72

[2]

CARMICHAEL LE, 1980, AM J VET RES, V41, P784

[3] DETECTION OF NORWALK VIRUS IN STOOL SPECIMENS BY REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION AND NONRADIOACTIVE OLIGOPROBES [J].

DELEON, R ;

MATSUI, SM ;

BARIC, RS ;

HERRMANN, JE ;

BLACKLOW, NR ;

GREENBERG, HB ;

SOBSEY, MD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (12) :3151-3157

[4] DETECTION OF ANIMAL AND HUMAN GROUP-B ROTAVIRUSES IN FECAL SPECIMENS BY POLYMERASE CHAIN-REACTION [J].

EIDEN, JJ ;

WILDE, J ;

FIROOZMAND, F ;

YOLKEN, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) :539-543

[5] POLYMERASE CHAIN-REACTION AMPLIFICATION AND TYPING OF ROTAVIRUS NUCLEIC-ACID FROM STOOL SPECIMENS [J].

GOUVEA, V ;

GLASS, RI ;

WOODS, P ;

TANIGUCHI, K ;

CLARK, HF ;

FORRESTER, B ;

FANG, ZY .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (02) :276-282

[6] MOLECULAR EPIDEMIOLOGY OF HUMAN HEPATITIS-A VIRUS DEFINED BY AN ANTIGEN-CAPTURE POLYMERASE CHAIN-REACTION METHOD [J].

JANSEN, RW ;

SIEGL, G ;

LEMON, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :2867-2871

[7]

JOHNSON R. H., 1967, J SMAL ANIM PRACT, V8, P319, DOI 10.1111/j.1748-5827.1967.tb04557.x

[8]

KUFFER M, 1995, TIERAERZTL PRAX, V23, P405

[9] RADIOIMMUNOFOCUS ASSAY FOR QUANTITATION OF HEPATITIS-A VIRUS IN CELL-CULTURES [J].

LEMON, SM ;

BINN, LN ;

MARCHWICKI, RH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 17 (05) :834-839

[10] COMPARISON OF POLYMERASE CHAIN-REACTION WITH VIRUS ISOLATION AND HEMAGGLUTINATION ASSAYS FOR THE DETECTION OF CANINE PARVOVIRUSES IN FECAL SPECIMENS [J].

MOCHIZUKI, M ;

SANGABRIEL, MC ;

NAKATANI, H ;

YOSHIDA, M .

RESEARCH IN VETERINARY SCIENCE, 1993, 55 (01) :60-63