A RAPID METHOD FOR PURIFICATION OF HERPESVIRUS DNA

被引：11

作者：

CHRISTENSEN, LS ^{[1
]}

NORMANN, P ^{[1
]}

机构：

[1] STATE VET INST VIRUS RES,KALVEHAVE,DENMARK

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 01期

关键词：

DNA PURIFICATION; HERPESVIRUS; MOLECULAR EPIDEMIOLOGY;

D O I：

10.1016/0166-0934(92)90024-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.

引用

页码：99 / 102

页数：4